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Figure 3: The graph showing non-human homologous essential genes encoding
different proteins involved in a same biological function in comparison with four
different strains.
These essential non-human homologous genes and their encoding protein were
further categorized on the basis of the pathways involved in the basic survival
mechanisms (Figure 3) such as: DNA replication, recombination, modification
and repair, translation and post translation modification, transport of small
molecule, transcription, RNA processing and degradation. These non-human
homologous gene their encoding protein were represented as ideal drug targets,
i.e, any disruption those genes may lead to bacterial death [Judson and
Mekalanos, 20001. These essential and non-human homologous genes cover 3-
4% of total genome of the organism. The MSA shows seven different types of
proteins are that are conserved among the strains and are essential non-human
homologues. These seven genes can be considered as a novel drug target to
design a drug Table 1. Among these seven genes Holliday junction DNA helicase
RuvB was taken as a target for ligand binding study.
Table 1: The predicted drug targets of Chlamydophila pneumoniae
Protein name
Excinuclease ABC subunit A
Holliday junction DNA
helicase RuvB
305 ribosomal protein S10
Function of protein
DNA Replication, Recombination,
modification and Repair
DNA Replication, Recombination,
modification and Repair
Translation ,post modification