4 - Central Institute of Brackishwater Aquaculture

National Workshop-cum-Training on Bioinformatlcs and Information Managemant in Aquaculture
Whole gmome squences from NCBl database
Screening of genes coding > 100 amino acids
hcdlction of essential genes with BLASTX against DEG database
Selection of essential and non-human homologous gene with BLASTS against Human genome
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1
Classification of protein squences on basis of biological function
by SWISSPORT database
Prediction of highily consaved protein squences common to all four C pnPumonzae strains
by using MSA (Multiple Squence Alignment) in ClustalX
I
Dmg targas
Figure 1: Insilico genomic approach for prediction of drug targets for
Chlamydophila pneumoniae.
2.1 Homology Modeling: The essential and non-human homologous common
protein sequence i.e Holliday junction DNA helicase RuvB protein was selected
from C. pneumoniae strains as a drug target [Tuteja et al., 2006; Frick, 20031.
Homology modeling is usually the method of choice when a clear relationship of
homology between the sequence of target protein and at least one known
structure is found. This approach would give reasonable results based on the
assumption that the tertiary structures of two proteins will be similar if their
sequences are related and three-dime5sional structure of proteins is better
conserved during evolution than its sequence [Kroemer et al., 1996; Rost,
19991. Building a homology model comprises four main steps: identification of
structural template(s), alignment of target sequence and template structure(s),
model building, and model quality evaluation. These steps can be repeated until
a satisfying modeling result is achieved. The query sequence Holliday junction
DNA helicase RuvB was searched to find out the related protein sequences as a
template by the BLAST program against the protein data bank (PDB). The
sequence that showed maximum identity with high score, better resolution,