an investigation into the antibacterial activities of medicinal plants ...

2.2.3.1 Bulbine frutescens 21
2.2.3.2 Leonotis leonurus 21
2.2.3.3 Melianthus major 22
2.2.3.4 Zantedeschia aethiopica 23
2.3 TOPICAL BURN WOUND INFECTIONS
2.3.1 Skin: Overview 25
2.3.2 Skin related infections 27
2.3.2.1 Wound infection and inflammation 27
2.3.2.2 Burn wound infection 29
2.3.2.3 Pathogens associated with burn wound infections 31
2.3.3 Bacteria selected for investigation 33
2.3.4 Antibiotic resistance 36
CHAPTER 3
METHODOLOGICAL JUSTIFICATION
3.1 Medicinal plants 38
3.2 Extraction of medicinal plants 39
3.3 Assays for antibacterial analysis 39
CHAPTER 4
METHODS AND MATERIALS
4.1 Plant selection and preparation 42
4.2 Plant extraction and traditional preparations 42
4.2.1 Plant extraction 42
4.2.2 Traditional preparations 45
4.3 Bacteria and growth conditions 45
4.4 Antibacterial assays
4.4.1 Microtitre plate assay 46
4.4.2 Agar dilution assay 49
4.4.2.1 Agar plate preparation 50
4.4.2.2 Agar plate inoculation 51
4.4.3 Standard agar plate count technique 52