an investigation into the antibacterial activities of medicinal plants ...

Plates supplemented with PNPG
No PNPG added
FIGURE 7
Reduced swarming of P. mirabilis on DST agar plates supplemented
with PNPG
A standard antibiotic plate, cotrimoxizole and control organisms (S. aureus, E.
coli, P. aeruginosa) were included as controls.
All the prepared plates were used on the day of preparation or stored
refrigerated (4 o C) for use within one week of preparation.
4.4.2.2 Agar plate inoculation
An inoculum for each bacterial strain was prepared by mixing colonies from
plate cultures (≤ 48 hrs) with 5 ml sterile distilled water and standardized to 0.5
McFarland density.
The test inocula were transferred into the sterile inoculum wells of the
multipoint-inoculating device (Mast diagnostics, UK), starting with the three
control bacterial suspensions followed by the test bacterial suspensions.
The extract plates were inoculated using the sterile inoculator pins of the
multipoint inoculator (Mast diagnostics) to transfer 1 - 2 µl of different inocula
onto agar plates containing extracts.
After inoculation plates were allowed to dry on the bench, before overnight
incubation at 37 o C.
Bacterial inhibition assessment was based on the analysis of growth on
control plates and the absence or presence of growth on the test extract
plates.
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