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an investigation into the antibacterial activities of medicinal plants ...

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S. aureus (S. a) P. aeruginosa (P. a)<br />

K. pneumoniae (K. p) S. a P. a K. p<br />

FIGURE 9<br />

Representations <strong>of</strong> MIC’s for acetone extracts <strong>of</strong> M. major in <strong>the</strong><br />

microtitre plates<br />

5.1.3 Antibacterial activity screening <strong>an</strong>d MIC determination<br />

A total <strong>of</strong> 73 bacterial strains were tested for <strong>an</strong>tibacterial activity using <strong>the</strong> microtitre<br />

plate assay <strong>an</strong>d <strong>the</strong> agar dilution assay. Thirty-two <strong>of</strong> <strong>the</strong> bacterial strains were<br />

Gram-positive <strong>an</strong>d 41 strains were Gram-negative. All 73 bacterial strains were<br />

initially screened for <strong>an</strong>tibacterial activity with <strong>the</strong> respective <strong>an</strong>tibacterial assays<br />

before <strong>the</strong> determination <strong>of</strong> <strong>the</strong> MIC’s <strong>of</strong> <strong>the</strong> pl<strong>an</strong>t extracts.<br />

Fifty-eight bacterial strains were screened with <strong>the</strong> microtitre plate assay <strong>an</strong>d 15<br />

bacterial strains were screened with <strong>the</strong> agar dilution assay. Table 6 indicates <strong>the</strong><br />

56

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