The obesogenic effects of polyunsaturated fatty acids are dependent ...

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with different carbohydrate sources ad-libitum for 8 weeks. (A) Body weight development is shown
as relative increase. (B) Energy efficiency was calculated as body weight gain divided by feed
intake. (C) Digestibility of energy, nitrogen, organic material and fat were calculated in a separate
set of mice (n=5). (D-F) Blood was collected, organs were dissected out and the weights were
recorded. Hormones and lipid parameters were measured in plasma and expressions of Acox1 (acyl-
CoA oxidase 1), Cpt1a (carnitine palmitoyltransferase 1a), Cpt2 (carnitine palmitoyltransferase 2),
Hmgcs2 (3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2), Srebf1 (sterol regulatory element
binding transcription factor 1), Scd1 (stearoyl-Coenzyme A desaturase 1), Acaca (acetyl-Coenzyme
A carboxylase alpha), Fasn (fatty acid synthase), Crem (cAMP responsive element modulator),
Pde4c (phosphodiesterase 4C, cAMP specific), Ppargc1a (peroxisome proliferator-activated
receptor gamma, coactivator 1 alpha), Pck1 (phosphoenolpyruvate carboxykinase 1, cytosolic),
Gpt1a (glutamic pyruvic transaminase 1a), Got1 (glutamate oxaloacetate transaminase 1, soluble),
Agxt (alanine-glyoxylate aminotransferase) and Cps1 (carbamoyl-phosphate synthetase 1) were
measured in liver using RT-qPCR and normalized to Tbp (TATA-box binding protein). Data are
presented as mean ± SEM. Different small letters denote significant differences (P