First 11 pages of thesis. - OPUS - Universität Würzburg

More documents

Recommendations

Info

Reagents Source For Immunohistochemistry Acetone J.T.Baker Hydrogen peroxide Sigma Blocking solution Dako ABC reagent Vector Laboratorties DAB reagent Vector Laboratorties For Mayer’s Haemalaun stain Haematoxylin Roth Sodium iodate Merck Aluminium calcium sulphate Merck Chloral hydrate Merck Citric acid Merck Antibodies Anti nitrotyrosine antibody Upstate Anti rabbit IgG Vector Laboratories Buffers and Solutions Krebs Hepes Buffer (KHB) KHB with the following composition, prepared in Milli Q water, was used for the experiments Sodium chloride 99 mM Potassium chloride 4.69 mM - 40 -
Calcium chloride 2.5 mM Magnesium sulphate 1.20 mM Sodium bicarbonate 25 mM Potassium dihydrogen phosphate 1.03 mM D (+) Glucose 5.6 mM Sodium Hepes 20 mM The above reagents were dissolved in 500 ml of MilliQ water. The pH was adjusted to 7.4. The pH was checked every day before use and the buffer was filtered using a 0.22 µm filter (Schleicher&Schuell). Phosphate Buffered Saline (PBS) 9.55 g of PBS were dissolved in 1 litre of distilled water to obtain a working solution of PBS. Mayer’s Haemalaun stain Haematoxylin 6 g Sodium iodate 1 g Aluminium calcium sulphate 250 g Chloral hydrate 250 g Citric acid 5 g All these reagents were dissolved in 5 liters of distilled water. - 41 -
Page 1 and 2: Insight into oxidative stress media
Page 3 and 4: Eidesstattliche Erklärungen Hiermi
Page 6 and 7: Acknowledgements First and foremost
Page 8 and 9: “Research is to see what everybod
Page 10 and 11: Chapter 2 37 2.0 Materials and Meth
Page 12 and 13: Summary and Hypothesis 76 Zusammenf
Page 14 and 15: 1.0 Introduction Atherosclerosis, t
Page 16 and 17: which contribute to the lipid rich
Page 18 and 19: 1.2 Risk Factors Atherosclerosis ha
Page 20 and 21: 1.2.2 Environmental factors 1. High
Page 22 and 23: 1.3 Oxidative Stress Oxidative stre
Page 24 and 25: peroxynitrite 38 which by itself ca
Page 26 and 27: Pharmacological inhibition of nitri
Page 28 and 29: 1.4 The nitric oxide pathway in ath
Page 30 and 31: of NADPH, FAD and FMN, whereas the
Page 32 and 33: isoforms to the regulatory and targ
Page 34 and 35: perinuclear/golgi region within the
Page 36 and 37: nNOS interacts with several protein
Page 38 and 39: iNOS expression is regulated transc
Page 40 and 41: transport pathway and is an essenti
Page 42 and 43: Interestingly, BH4 supplementation
Page 44 and 45: the survival rate, as apoE/nNOS dko
Page 46 and 47: The seemingly opposing effects of i
Page 48 and 49: The purpose of the study presented
Page 50 and 51: 2.0 Materials and Methods 2.1 Mater
Page 54 and 55: Others Reagents Source Pegulated Su
Page 56 and 57: 2.2 Methods 2.2.1 Detection of free
Page 58 and 59: 2.2.1.2 Principle of ESR ESR spectr
Page 60 and 61: spectra 190 . Addition of exogenous
Page 62 and 63: ings were prepared as mentioned abo
Page 64 and 65: X-band EMX spectroscope (Bruker Bio
Page 66 and 67: minutes. At last sections were wash
Page 68 and 69: 3.0 Results 3.1 eNOS is a significa
Page 70 and 71: 3.2 eNOS deletion decreases vascula
Page 72 and 73: 3.3 nNOS contributes little to vasc
Page 74 and 75: 3.5 iNOS contributes significantly
Page 76 and 77: Figure 13. Determination of iNOS de
Page 78 and 79: 3.8 iNOS deletion influences NADPH
Page 80 and 81: 4.0 Discussion 4.1 eNOS is a major
Page 82 and 83: in our apoE/eNOS dko model, these a
Page 84 and 85: under conditions of substrate or co
Page 86 and 87: vessels with the iNOS specific inhi
Page 88 and 89: Summary and Hypothesis The principl
Page 90 and 91: Zusammenfassung und Hypothese Stick
Page 92 and 93: Bibliography 1. Gimbrone MA, Jr. Va
Page 94 and 95: xanthine oxidase contributes to vas
Page 96 and 97: expression of adhesion molecules an
Page 98 and 99: transforming growth factor-beta 1.
Page 100 and 101: generation from neuronal nitric oxi
Page 102 and 103:
148. Lee PC, Wang ZL, Qian S, Watki
Page 104 and 105:
174. Mayr U, Zou Y, Zhang Z, Dietri
Page 106 and 107:
implications in intracellular fluor
Page 108 and 109:
Definitions Thesaurus • Allograft
Page 110 and 111:
• Claudication refers to leg pain
Page 112 and 113:
cases per year in the US. It is a n
Page 114 and 115:
Curriculum Curriculum vitae vitae N
Page 116 and 117:
presentation at the 37 th Annual co
show all

First 11 pages of thesis. - OPUS - Universität Würzburg

Create successful ePaper yourself

Delete template?

Save as template?