First 11 pages of thesis. - OPUS - Universität Würzburg

Figure 9. Determination of eNOS derived superoxide production: a) HPLC measurements
of superoxide formation showed significantly lower levels of superoxide production in
apoE/eNOS dko, compared to apoE ko mice following 18 weeks of western diet. Hyperlipidemic
apoE ko and apoE/eNOS dko mice had significantly higher superoxide levels, compared to
normocholesterolemic C57Bl6 animals (*p=0.000001; § p=0.02; # p=0.04). b) Unselective NOS
inhibition by L-NAME resulted in inhibition of superoxide production in apoE ko mice
(*p=0.00001). c) Vascular superoxide production from 24 weeks western diet fed apoE ko mice
were significantly elevated, compared to the 18 weeks time point (*p=0.05). Additionally,
treatment of apoE ko vessel rings with the eNOS specific inhibitor L-NIO decreased vascular
superoxide formation in 24 weeks western diet fed apoE ko animals ( § p=0.02).
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