First 11 pages of thesis. - OPUS - Universität Würzburg
First 11 pages of thesis. - OPUS - Universität Würzburg
First 11 pages of thesis. - OPUS - Universität Würzburg
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Calcium chloride 2.5 mM<br />
Magnesium sulphate 1.20 mM<br />
Sodium bicarbonate 25 mM<br />
Potassium dihydrogen phosphate 1.03 mM<br />
D (+) Glucose 5.6 mM<br />
Sodium Hepes 20 mM<br />
The above reagents were dissolved in 500 ml <strong>of</strong> MilliQ water. The pH<br />
was adjusted to 7.4. The pH was checked every day before use and the buffer<br />
was filtered using a 0.22 µm filter (Schleicher&Schuell).<br />
Phosphate Buffered Saline (PBS)<br />
9.55 g <strong>of</strong> PBS were dissolved in 1 litre <strong>of</strong> distilled water to obtain a<br />
working solution <strong>of</strong> PBS.<br />
Mayer’s Haemalaun stain<br />
Haematoxylin 6 g<br />
Sodium iodate 1 g<br />
Aluminium calcium sulphate 250 g<br />
Chloral hydrate 250 g<br />
Citric acid 5 g<br />
All these reagents were dissolved in 5 liters <strong>of</strong> distilled water.<br />
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