Amit Alexander Charan and Abhishek Sharan1980; Dougal 1981; Coll<strong>in</strong>s, 1987). Progress <strong>in</strong> medic<strong>in</strong>al plant research has undergonea phenomenal growth dur<strong>in</strong>g last two decades. Worldwide trend towards the utilization<strong>of</strong> natural plant remedies has created an enormous need for <strong>in</strong>formation about theproperties and uses <strong>of</strong> medic<strong>in</strong>al plants as antitumor, antianalgesic, <strong>in</strong>secticides (Jacobson,1975), rotenoides etc. Demand on plant based therapeutics has <strong>in</strong>creased many foldbecause they are natural products hav<strong>in</strong>g no side effects and easily available at affordableprices (Govil et al., 2002). The goal <strong>of</strong> present work is to analyze primary metaboliteswhich are directly concerned with metabolic processes like - respiration, photosynthesis,prote<strong>in</strong>, lipid synthesis.MATERIALS AND METHODSIn the proposed work different species were collected from School <strong>of</strong> Forestry &Environment, Sam Higg<strong>in</strong>bottom Institute <strong>of</strong> Agriculture, Technology & Sciences,Allahabad. Fresh leaves were washed and dried <strong>in</strong> an <strong>in</strong>cubator at 37 0 C and make itspowder. This powder was used for further analyses <strong>of</strong> Total Soluble Sugar and Chlorophyll(Sadasivam and Manickam, 1992), Prote<strong>in</strong> (Lowry et al., 1951), Total Lipids (Blighand Dyer, 1959), Total Phenols (Bray and Thorpe, 1954), Starch (Dubois et al.,1951).RESULTS AND DISCUSSIONHigher amount <strong>of</strong> sugar was observed <strong>in</strong> the Cymbopogan citratus i.e. 3.12%followed by Coriandrum sativum i.e. 2.880 as compared to other plants while lowestwas obta<strong>in</strong>ed from Aloe vera i.e. 2.872%. Highest content <strong>of</strong> chlorophyll was observed<strong>in</strong> leafs <strong>of</strong> Coriandrum sativum i.e. 0.670% followed by Cymbopogan citratus i.e.Table 1: Comparative analysis <strong>of</strong> primary metabolites <strong>in</strong> medic<strong>in</strong>al plants.Amount <strong>of</strong> ConstituentsName <strong>of</strong> plant Soluble Chlorophyll Prote<strong>in</strong> Lipids Phenols Starchsugar(%) (%) (%) (%) (%) (%)Aloe vera 2.872 0.613 3.380 1.00 0.050 3.060Cymbopogan 3.120 0.614 3.592 7.08 0.063 3.492citratusCoriandrum 2.880 0.670 2.544 0.370 0.061 3.096sativum114
The Allahabad Farmer Vol. LXVIII, January - 2013 No. 20.614% while lowest was obta<strong>in</strong>ed from Aloe vera i.e. 0.613%. Maximum amount <strong>of</strong>prote<strong>in</strong> was estimated <strong>in</strong> Cymbopogan citratus i.e. 3.592% followed by Aloe vera i.e.3.380% and lowest was obta<strong>in</strong>ed from Coriandrum sativum i.e. 2.544%. Highest amount<strong>of</strong> lipids was calculated <strong>in</strong> Cymbopogan citratus i.e. 7.08% followed by Aloe vera i.e.1.0% and lowest was obta<strong>in</strong>ed from Coriandrum sativum i.e. 0.370%. Maximum amount<strong>of</strong> phenols was found <strong>in</strong> Cymbopogan citratus i.e. 0.063% and followed by Coriandrumsativum i.e. 0.061% while lowest was found from Aloe vera i.e. 0.050% and starch wasestimated as 3.492% <strong>in</strong> Cymbopogan citratus and followed by Coriandrum sativumi.e. 3.096% and lowest was calculated <strong>in</strong> Aloe vera i.e. 3.060% (Table 1).REFERENCESBligh, E.G. and Dyer, W.J. (1959). Biochemical methods <strong>of</strong> Plant Physiology. Journal<strong>of</strong> Biochemistry and Physiology. 37: 911.Bray, H.G. and Thorpe, W.V. (1954). Biochemical estimation for Phenolic compounds.Methods <strong>of</strong> Biochemical Analysis. 1: 27-52.Coll<strong>in</strong>s, H.A. (1987). Determ<strong>in</strong>ants <strong>of</strong> yield <strong>of</strong> secondary products <strong>in</strong> plants tissuecultures. Advances <strong>in</strong> Botanical Research. 13: 145-87.Despande, V.V., Mendular, K.N. and Sadre, N.L. (1980). Antifertility activity<strong>of</strong> Azadirachta <strong>in</strong>dica <strong>in</strong> mice. Journal <strong>of</strong> Postgraduation Med<strong>in</strong>ce.26: 167-170.Dougal, D.K. (1981). Tissue culture and the study <strong>of</strong> secondary (natural) products.Biochemistry <strong>in</strong> Plants. 7: 21-34.Govil, J.N., S<strong>in</strong>gh, V.K. and S<strong>in</strong>gh, G. (2002). Recent progress <strong>in</strong> medic<strong>in</strong>al plants.Ethanomedic<strong>in</strong>e and Pharmacognosy. 1: 37-51.Jacobson, M. (1975). Insecticides from plants a review <strong>of</strong> literature 1954-1957.Agricultural Handbook 461 U.S. Department <strong>of</strong> Agriculture, AgriculturalResearch Services, Wash<strong>in</strong>gton D.C.Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). Method <strong>of</strong>prote<strong>in</strong> estimation. Journal <strong>of</strong> Biological Chemistry. 193: 265.Ray, A.B., Sharma, B.K., and S<strong>in</strong>gh, U.P. (2004). Medic<strong>in</strong>al properties <strong>of</strong> plants.International Book Distribut<strong>in</strong>g Co.Sadasivam, S. and Manickam, A. (1992). In: Biochemical Methods for AgriculturalSciences, Wiley Eastern Limited, New Delhi, pp. 184-185.115
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