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Study of Hygienic practices of street food vendors in ... - Shiats.edu.in

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Seweta Srivastava, Asha S<strong>in</strong>haThe first count for germ<strong>in</strong>ated seeds was taken 5 days and the second 7 days afterplat<strong>in</strong>g, respectively.Determ<strong>in</strong>ation <strong>of</strong> Moisture content <strong>of</strong> Fresh and Stored Jatropha Seeds :About 5 to 6 g Jatropha curcas seeds were accurately weighed <strong>in</strong> a Petri dish andkept <strong>in</strong> hot-air oven ma<strong>in</strong>ta<strong>in</strong>ed at 110 o C for 4 hours. After cool<strong>in</strong>g <strong>in</strong> a dessicator, theloss <strong>in</strong> weight was recorded <strong>in</strong> each case. This proc<strong>edu</strong>re was repeated till constantweight was obta<strong>in</strong>ed (Nayak and Patel, 2010). The moisture content <strong>of</strong> seeds wasdeterm<strong>in</strong>ed with the help <strong>of</strong> the formula given below:Initial weight <strong>of</strong> seeds - Weight <strong>of</strong> seeds after dry<strong>in</strong>gMoisture content <strong>of</strong> seeds (%) = -------------------------------------------------------------------------- X 100Initial weight <strong>of</strong> seedsStatistical Analysis: Mean value with standard error was calculated to check the variation<strong>in</strong> per cent germ<strong>in</strong>ation and moisture content <strong>of</strong> Jatropha curcas L. seeds dur<strong>in</strong>g oneyear <strong>of</strong> storage. The term 'Standard Error' <strong>of</strong> any estimate is used for a measure <strong>of</strong> theaverage magnitude <strong>of</strong> the difference between the sample estimate and the populationparameter taken over all possible samples <strong>of</strong> the same size, from the population (Chandel,2002).RESULTS AND DISCUSSIONData presented <strong>in</strong> Table-I reveal that fresh Jatropha seeds showed maximumgerm<strong>in</strong>ation and <strong>in</strong> storage percent germ<strong>in</strong>ation was decreased. After 3 months,maximum germ<strong>in</strong>ation was shown by control by us<strong>in</strong>g blotter method i.e., 90% followedby multi-pot tray method i.e., 88.16% and m<strong>in</strong>imum germ<strong>in</strong>ation was shown byPenicillium rubrum <strong>in</strong>fested seeds <strong>in</strong> earthen pot i.e., 66.67%. After 6 months, maximumgerm<strong>in</strong>ation was shown by control <strong>in</strong> earthen pot i.e., 83.33% followed by multi-pottray method i.e., 80.26% and m<strong>in</strong>imum germ<strong>in</strong>ation was shown by Penicillium rubrum<strong>in</strong>fested seeds <strong>in</strong> earthen pot i.e., 62.50%. After 9 months, maximum germ<strong>in</strong>ation wasshown by control <strong>in</strong> earthen pot i.e., 75% followed by multipot tray method i.e., 73.68%and m<strong>in</strong>imum germ<strong>in</strong>ation was shown by Aspergillus fumigatus, Penicillium rubrumand Fusarium chlamydosporum <strong>in</strong>fested seeds <strong>in</strong> earthen pot and <strong>in</strong> blotter method i.e.,50%. After 12 months, maximum germ<strong>in</strong>ation was shown by control <strong>in</strong> multiport tray i.e.,71.05% followed by earthen pot method i.e., 66.67% and m<strong>in</strong>imum germ<strong>in</strong>ation wasshown by Aspergillus fumigatus Fusarium chlamydosporum <strong>in</strong>fested seeds <strong>in</strong> blottermethod i.e., 30%.66

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