Advanced Techniques in Diagnostic Microbiology

Index
A
acetate utilization, 100
Acinetobacter spp.
blood cultures, 4
pulsed-field gel electrophoresis, 149, 151
strain typing, 453
susceptibility testing, 64
Actinomyces, 16S rRNA, 328
adenovirus
in-situ hybridization, 139
multiplexed bead assay, 56
rapid antigen tests, 34, 35
advanced antibody detection
automation, 57–58
clinical applications, 53–57
ideal characteristics, 43
immunoblotting methods, 46
principles, 42–50
technique characteristics, 42–50
techniques compared, 50–53
See also specific methods; organisms
Aerococcus
trypticase broth, 95
vancomycin disk test, 91
Aeromonas, 102
Affirm DNA Probe, 137–138
AFLP. See amplified fragment-length
polymorphism
agar proportion method, 399
agarose gel electrophoresis
cross-method comparisons, 258–259
gel performance, 244–246
principles, 244
target detection, 246–248
agglutination methods
prozone reaction, 23
rapid antigen tests, 32–33, 35
rheumatoid factor antibodies, 25
technique characteristics, 29–30
Amplicor test, 396
amplification product inactivation methods. See
inactivation methods
amplified fragment-length polymorphism
(AFLP)
organism-specific applications, 450
strain typing, 451
anaerobic bacteria, growth conditions, 71
antimicrobial susceptibility testing
advantages, 63
agar dilution, 64–65
automated methods, 68–69
automation, 70
broth dilution, 65–66
calcium-magnesium supplements, 64
categories, 63
clinician experience levels, 66
CLSI methods, 70
detection of specific mechanisms, 72
diffusion tests, 63, 66–68
dilution tests, 63
E-test, 67–68
fastidious organisms, 71–72
inoculation sizes, 64
labor intensity, 66
microdilution testing methods, 68–69
AP-PCR. See arbitrarily primed PCR
API identification system, 104–105
arbitrarily primed PCR (AP-PCR),
177–179
Arcanobacterium haemolyticum, 94
Aspergillus spp.
environmental monitoring, 457–459
pulsed-field gel electrophoresis, 149
astrovirus, 35
525