Advanced Techniques in Diagnostic Microbiology

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416 P. Francois and J. Schrenzel A S.aureus B C D FIGURE 24.2. Illustrative examples of chromogenic media. (A). ORSAB plates (Oxoid) contain oxacillin and a tracer of mannitol fermentation yielding blue Staphylococcus aureus colonies. (B). Selective ChromAgar plate (ChromAgar), yielding pink Staphylococcus aureus colonies (pictures are visible on the respective Web sites of the companies). Standard mannitol–salt agar plate showing colony aspects resulting from negative (C) or positive (D) MRSA cultures. [See Color Plate II]
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Advanced Techniques in Diagnostic M
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Yi-Wei Tang Molecular Infectious Di
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vi Contributors Wonder Drake Depart
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viii Contributors Jacques Schrenzel
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Preface Clinical microbiologists ar
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Contents Part I Techniques 1 Automa
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Contents xv 22 Review of Molecular
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1 Automated Blood Cultures XIANG Y.
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Interpretation of Significance 1. A
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1. Automated Blood Cultures 7 conta
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1. Automated Blood Cultures 9 Crump
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2 Urea Breath Tests for Detection o
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2. Urea Breath Tests 13 and point o
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2. Urea Breath Tests 15 Other detec
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TABLE 2.1. Comparison of 13 C-urea
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2. Urea Breath Tests 19 Bazzoli, F.
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2. Urea Breath Tests 21 Logan, R. (
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3 Rapid Antigen Tests SHELDON CAMPB
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3. Rapid Antigen Tests 25 antigen i
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3. Rapid Antigen Tests 27 either on
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3. Rapid Antigen Tests 29 and moves
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3. Rapid Antigen Tests 31 Applicati
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Fungi Cryptococcus Agglutination, E
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Adenovirus, enteric types 40,41 EIA
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3. Rapid Antigen Tests 37 Antigen t
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3. Rapid Antigen Tests 39 retains a
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3. Rapid Antigen Tests 41 Wilhelmi,
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4. Advanced Antibody Detection 43 D
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TABLE 4.1. Types of antibody detect
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4. Advanced Antibody Detection 47 c
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4. Advanced Antibody Detection 49 U
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4. Advanced Antibody Detection 51 a
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4. Advanced Antibody Detection 53 a
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4. Advanced Antibody Detection 55 H
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4. Advanced Antibody Detection 57 r
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4. Advanced Antibody Detection 59 A
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4. Advanced Antibody Detection 61 M
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5 Phenotypic Testing of Bacterial A
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5. Antimicrobial Susceptibility Tes
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Susceptibility Tests for Fastidious
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References 5. Antimicrobial Suscept
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6. Biochemical Profile-Based Microb
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7 Rapid Bacterial Characterization
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7. MALDI-TOF MS 119 photons followe
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7. MALDI-TOF MS 121 for analysis by
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7. MALDI-TOF MS 123 Sample wells Ca
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7. MALDI-TOF MS 125 also given. Thi
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7. MALDI-TOF MS 127 characteristics
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References 7. MALDI-TOF MS 129 Ande
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7. MALDI-TOF MS 131 Hindre, T., Did
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7. MALDI-TOF MS 133 von Wintzingero
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8. Probe-Based Microbial Detection
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9 Pulsed-Field Gel Electrophoresis
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9. Pulsed-Field Gel Electrophoresis
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PFGE Performance Characteristics 9.
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TABLE 10.1. Nucleic acid amplificat
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10. In Vitro Nucleic Acid Amplifica
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11. PCR and Its Variations 167 Prin
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11. PCR and Its Variations 169 Exte
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11. PCR and Its Variations 171 targ
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11. PCR and Its Variations 173 bind
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11. PCR and Its Variations 175 Reve
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11. PCR and Its Variations 177 ampl
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11. PCR and Its Variations 179 pres
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11. PCR and Its Variations 181 Soft
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11. PCR and Its Variations 183 Saik
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12. Non-PCR Amplification 185 1989;
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12. Non-PCR Amplification 187 FIGUR
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12. Non-PCR Amplification 189 This
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12. Non-PCR Amplification 191 FIGUR
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12. Non-PCR Amplification 193 and c
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12. Non-PCR Amplification 195 used
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12. Non-PCR Amplification 197 do no
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Application of the SDA Technique Re
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12. Non-PCR Amplification 201 Ancho
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12. Non-PCR Amplification 203 Baner
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12. Non-PCR Amplification 205 Guilf
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12. Non-PCR Amplification 207 Nilss
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12. Non-PCR Amplification 209 Wang,
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13. Recent Advances in Probe Amplif
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14. Signal Amplification Techniques
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TABLE 14.1. Comparison of bDNA and
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References 14. Signal Amplification
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15 Detection and Characterization o
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15. Agarose Gel Electrophoresis, So
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16. Direct Nucleotide Sequencing 26
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17. Microarrays for Microbial Chara
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18 Diagnostic Microbiology Using Re
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TABLE 18.1. Comparison of four fluo
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18. Real-Time PCR Based on FRET 295
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19. Amplification Product Inactivat
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TABLE 19.1. Comparison of inactivat
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20 Bacterial Identification Based o
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20. Analysis of 16S rRNA Gene Seque
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21 Molecular Techniques for Blood a
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Hepatitis B Surface Antigen (Anti-H
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Vironostika HIV-1 Microelisa System
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21. Blood and Blood Product Screeni
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22 Review of Molecular Techniques f
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22. Molecular Techniques for STDs D
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Traditional Diagnostic Methods 22.
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References 22. Molecular Techniques
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23 Advances in the Diagnosis of Myc
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23. Diagnosis of Mycobacterium tube
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Page 842: 24 Rapid Screening and Identificati
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Page 860: 420 P. Francois and J. Schrenzel Ac
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Page 872: 426 P. Francois and J. Schrenzel Va
Page 876: 428 D. Ernst et al. several microme
Page 880: 430 D. Ernst et al. reanalysis to d
Page 884: 432 D. Ernst et al. Neisseria menin
Page 888: 434 D. Ernst et al. A. B. SEB-beads
Page 892: 436 D. Ernst et al. IL-6, IL-8, and
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442 D. Ernst et al. Lal, G., Balmer
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26 Molecular Strain Typing Using Re
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446 S. R. Frye and M. Healy FIGURE
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448 S. R. Frye and M. Healy Automat
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450 S. R. Frye and M. Healy TABLE 2
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452 S. R. Frye and M. Healy Noncomm
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454 S. R. Frye and M. Healy 2001).
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456 S. R. Frye and M. Healy (Stempe
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458 S. R. Frye and M. Healy isolate
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460 S. R. Frye and M. Healy molecul
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462 S. R. Frye and M. Healy Boerlin
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464 S. R. Frye and M. Healy Fey, P.
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466 S. R. Frye and M. Healy Kwara,
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468 S. R. Frye and M. Healy Pfaller
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470 S. R. Frye and M. Healy Tang, Y
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27 Molecular Differential Diagnoses
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474 J. Han The technology advanceme
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476 J. Han Targets Optimal Conditio
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478 J. Han TABLE 27.1. Comparison o
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480 J. Han TABLE 27.3. Comparison o
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482 J. Han suspension hybridization
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484 J. Han fluorescent dye is remov
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486 J. Han TABLE 27.5. List of path
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TABLE 27.8. Detection results of th
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490 J. Han common in HAI. In additi
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492 J. Han TABLE 27.12. Detection r
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494 J. Han TABLE 27.14. List of pat
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496 J. Han TABLE 27.18. Detectable
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TABLE 27.20. List of gene targets i
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500 J. Han Benefits and Impact: Del
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502 J. Han significantly increasing
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504 J. Han Hindiyeh, M., Hillyard D
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506 E. M. Marlowe and D. M. Wolk Al
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TABLE 28.1. Automated specimen proc
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510 E. M. Marlowe and D. M. Wolk Au
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512 E. M. Marlowe and D. M. Wolk La
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514 E. M. Marlowe and D. M. Wolk me
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516 E. M. Marlowe and D. M. Wolk mo
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518 E. M. Marlowe and D. M. Wolk Mo
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520 E. M. Marlowe and D. M. Wolk Ha
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522 E. M. Marlowe and D. M. Wolk Sa
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Index A acetate utilization, 100 Ac
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Index 527 rapid antigen tests, 27-2
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Index 529 flow cytometric immunoass
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Index 531 Human T-Lymphotropic Viru
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Index 533 MLEE. See multilocus enzy
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Index 535 PNA. See peptide-nucleic
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Index 537 Roseomonas, 328-329 rotav
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Index 539 T TaqMan probes, 293-296
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