Advanced Techniques in Diagnostic Microbiology

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352 Y. Hu and I. Hirshfield Tang, Y.W., Procop, G.W., & Persing, D.H. (1997). Molecular diagnostics of infectious diseases. Clin Chem, 43, 2021–2038. U.S. Food & Drug Administration. (2004). Testing requirements for communicable disease agents. Federal Register, 21CFR 610:40, 75–80. Wang, Q.M., & Heinz, B.A. (2001). Recent advances in prevention and treatment of hepatitis C virus infections. Progress Drug Res, Spec, 79–110. Yu, X., Susa, M., Knabbe, C., Schmid, R.D., & Bachmann, T.T. (2004). Development and validation of a diagnostic DNA microarray to detect quinoloneresistant Escherichia coli among clinical isolates. J Clin Microbiol, 42, 4083–4091.
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Advanced Techniques in Diagnostic M
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Yi-Wei Tang Molecular Infectious Di
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vi Contributors Wonder Drake Depart
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viii Contributors Jacques Schrenzel
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Preface Clinical microbiologists ar
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Contents Part I Techniques 1 Automa
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Contents xv 22 Review of Molecular
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1 Automated Blood Cultures XIANG Y.
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Interpretation of Significance 1. A
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1. Automated Blood Cultures 7 conta
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1. Automated Blood Cultures 9 Crump
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2 Urea Breath Tests for Detection o
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2. Urea Breath Tests 13 and point o
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2. Urea Breath Tests 15 Other detec
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TABLE 2.1. Comparison of 13 C-urea
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2. Urea Breath Tests 19 Bazzoli, F.
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2. Urea Breath Tests 21 Logan, R. (
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3 Rapid Antigen Tests SHELDON CAMPB
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3. Rapid Antigen Tests 25 antigen i
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3. Rapid Antigen Tests 27 either on
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3. Rapid Antigen Tests 29 and moves
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3. Rapid Antigen Tests 31 Applicati
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Fungi Cryptococcus Agglutination, E
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Adenovirus, enteric types 40,41 EIA
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3. Rapid Antigen Tests 37 Antigen t
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3. Rapid Antigen Tests 39 retains a
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3. Rapid Antigen Tests 41 Wilhelmi,
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4. Advanced Antibody Detection 43 D
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TABLE 4.1. Types of antibody detect
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4. Advanced Antibody Detection 47 c
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4. Advanced Antibody Detection 49 U
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4. Advanced Antibody Detection 51 a
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4. Advanced Antibody Detection 53 a
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4. Advanced Antibody Detection 55 H
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4. Advanced Antibody Detection 57 r
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4. Advanced Antibody Detection 59 A
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4. Advanced Antibody Detection 61 M
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5 Phenotypic Testing of Bacterial A
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5. Antimicrobial Susceptibility Tes
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Susceptibility Tests for Fastidious
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References 5. Antimicrobial Suscept
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6. Biochemical Profile-Based Microb
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7 Rapid Bacterial Characterization
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7. MALDI-TOF MS 119 photons followe
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7. MALDI-TOF MS 121 for analysis by
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7. MALDI-TOF MS 123 Sample wells Ca
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7. MALDI-TOF MS 125 also given. Thi
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7. MALDI-TOF MS 127 characteristics
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References 7. MALDI-TOF MS 129 Ande
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7. MALDI-TOF MS 131 Hindre, T., Did
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7. MALDI-TOF MS 133 von Wintzingero
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8. Probe-Based Microbial Detection
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9 Pulsed-Field Gel Electrophoresis
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9. Pulsed-Field Gel Electrophoresis
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PFGE Performance Characteristics 9.
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TABLE 10.1. Nucleic acid amplificat
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10. In Vitro Nucleic Acid Amplifica
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11. PCR and Its Variations 167 Prin
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11. PCR and Its Variations 169 Exte
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11. PCR and Its Variations 171 targ
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11. PCR and Its Variations 173 bind
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11. PCR and Its Variations 175 Reve
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11. PCR and Its Variations 177 ampl
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11. PCR and Its Variations 179 pres
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11. PCR and Its Variations 181 Soft
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11. PCR and Its Variations 183 Saik
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12. Non-PCR Amplification 185 1989;
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12. Non-PCR Amplification 187 FIGUR
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12. Non-PCR Amplification 189 This
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12. Non-PCR Amplification 191 FIGUR
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12. Non-PCR Amplification 193 and c
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12. Non-PCR Amplification 195 used
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12. Non-PCR Amplification 197 do no
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Application of the SDA Technique Re
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12. Non-PCR Amplification 201 Ancho
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12. Non-PCR Amplification 203 Baner
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12. Non-PCR Amplification 205 Guilf
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12. Non-PCR Amplification 207 Nilss
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12. Non-PCR Amplification 209 Wang,
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13. Recent Advances in Probe Amplif
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14. Signal Amplification Techniques
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TABLE 14.1. Comparison of bDNA and
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References 14. Signal Amplification
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15 Detection and Characterization o
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15. Agarose Gel Electrophoresis, So
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16. Direct Nucleotide Sequencing 26
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17. Microarrays for Microbial Chara
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18 Diagnostic Microbiology Using Re
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TABLE 18.1. Comparison of four fluo
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18. Real-Time PCR Based on FRET 295
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19. Amplification Product Inactivat
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TABLE 19.1. Comparison of inactivat
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20 Bacterial Identification Based o
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20. Analysis of 16S rRNA Gene Seque
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378 A. C. T. Lo and K. M. Kam Hippe
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380 A. C. T. Lo and K. M. Kam Larse
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382 A. C. T. Lo and K. M. Kam Nobbe
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384 A. C. T. Lo and K. M. Kam chlam
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386 A. C. T. Lo and K. M. Kam ureal
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388 A. Kilic and W. Drake Lipids wi
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390 A. Kilic and W. Drake America,
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392 A. Kilic and W. Drake MTB from
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394 A. Kilic and W. Drake with anti
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396 A. Kilic and W. Drake Molecular
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398 A. Kilic and W. Drake and monit
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400 A. Kilic and W. Drake system; t
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402 A. Kilic and W. Drake transillu
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404 A. Kilic and W. Drake Real-Time
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406 A. Kilic and W. Drake Caws, M.,
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408 A. Kilic and W. Drake Marttila,
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410 A. Kilic and W. Drake Torres, M
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412 P. Francois and J. Schrenzel an
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414 P. Francois and J. Schrenzel th
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416 P. Francois and J. Schrenzel A
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418 P. Francois and J. Schrenzel St
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420 P. Francois and J. Schrenzel Ac
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422 P. Francois and J. Schrenzel or
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424 P. Francois and J. Schrenzel Lo
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426 P. Francois and J. Schrenzel Va
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428 D. Ernst et al. several microme
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430 D. Ernst et al. reanalysis to d
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432 D. Ernst et al. Neisseria menin
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434 D. Ernst et al. A. B. SEB-beads
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436 D. Ernst et al. IL-6, IL-8, and
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pg/ml 120 100 80 60 40 20 0 120 100
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440 D. Ernst et al. Although interf
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442 D. Ernst et al. Lal, G., Balmer
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26 Molecular Strain Typing Using Re
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446 S. R. Frye and M. Healy FIGURE
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448 S. R. Frye and M. Healy Automat
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450 S. R. Frye and M. Healy TABLE 2
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452 S. R. Frye and M. Healy Noncomm
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454 S. R. Frye and M. Healy 2001).
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456 S. R. Frye and M. Healy (Stempe
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458 S. R. Frye and M. Healy isolate
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460 S. R. Frye and M. Healy molecul
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462 S. R. Frye and M. Healy Boerlin
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464 S. R. Frye and M. Healy Fey, P.
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466 S. R. Frye and M. Healy Kwara,
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468 S. R. Frye and M. Healy Pfaller
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470 S. R. Frye and M. Healy Tang, Y
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27 Molecular Differential Diagnoses
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474 J. Han The technology advanceme
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476 J. Han Targets Optimal Conditio
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478 J. Han TABLE 27.1. Comparison o
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480 J. Han TABLE 27.3. Comparison o
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482 J. Han suspension hybridization
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484 J. Han fluorescent dye is remov
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486 J. Han TABLE 27.5. List of path
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TABLE 27.8. Detection results of th
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490 J. Han common in HAI. In additi
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492 J. Han TABLE 27.12. Detection r
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494 J. Han TABLE 27.14. List of pat
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496 J. Han TABLE 27.18. Detectable
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TABLE 27.20. List of gene targets i
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500 J. Han Benefits and Impact: Del
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502 J. Han significantly increasing
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504 J. Han Hindiyeh, M., Hillyard D
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506 E. M. Marlowe and D. M. Wolk Al
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TABLE 28.1. Automated specimen proc
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510 E. M. Marlowe and D. M. Wolk Au
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512 E. M. Marlowe and D. M. Wolk La
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514 E. M. Marlowe and D. M. Wolk me
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516 E. M. Marlowe and D. M. Wolk mo
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518 E. M. Marlowe and D. M. Wolk Mo
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520 E. M. Marlowe and D. M. Wolk Ha
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522 E. M. Marlowe and D. M. Wolk Sa
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Index A acetate utilization, 100 Ac
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Index 527 rapid antigen tests, 27-2
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Index 529 flow cytometric immunoass
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Index 531 Human T-Lymphotropic Viru
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Index 533 MLEE. See multilocus enzy
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Index 535 PNA. See peptide-nucleic
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Index 537 Roseomonas, 328-329 rotav
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Index 539 T TaqMan probes, 293-296
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