Advanced Techniques in Diagnostic Microbiology

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540 Index W West Nile (WN) virus, 56, 346–347 ELISA assays, 54 MDDs, 492 See also blood screening Western blot, 46, 54 whole community genome amplification (WCGA), 284 whole-genome analysis, 185 WN. See West Nile virus Wuchereria bancrofti, 38 X xMAP. See multianalytic profile Y yeasts blood cultures, 6–7 ITS regions, 269 Yersinia pestis susceptibility testing, 64
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Advanced Techniques in Diagnostic M
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Yi-Wei Tang Molecular Infectious Di
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vi Contributors Wonder Drake Depart
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viii Contributors Jacques Schrenzel
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Preface Clinical microbiologists ar
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Contents Part I Techniques 1 Automa
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Contents xv 22 Review of Molecular
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1 Automated Blood Cultures XIANG Y.
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Interpretation of Significance 1. A
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1. Automated Blood Cultures 7 conta
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1. Automated Blood Cultures 9 Crump
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2 Urea Breath Tests for Detection o
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2. Urea Breath Tests 13 and point o
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2. Urea Breath Tests 15 Other detec
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TABLE 2.1. Comparison of 13 C-urea
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2. Urea Breath Tests 19 Bazzoli, F.
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2. Urea Breath Tests 21 Logan, R. (
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3 Rapid Antigen Tests SHELDON CAMPB
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3. Rapid Antigen Tests 25 antigen i
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3. Rapid Antigen Tests 27 either on
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3. Rapid Antigen Tests 29 and moves
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3. Rapid Antigen Tests 31 Applicati
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Fungi Cryptococcus Agglutination, E
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Adenovirus, enteric types 40,41 EIA
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3. Rapid Antigen Tests 37 Antigen t
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3. Rapid Antigen Tests 39 retains a
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3. Rapid Antigen Tests 41 Wilhelmi,
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4. Advanced Antibody Detection 43 D
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TABLE 4.1. Types of antibody detect
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4. Advanced Antibody Detection 47 c
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4. Advanced Antibody Detection 49 U
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4. Advanced Antibody Detection 51 a
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4. Advanced Antibody Detection 53 a
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4. Advanced Antibody Detection 55 H
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4. Advanced Antibody Detection 57 r
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4. Advanced Antibody Detection 59 A
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4. Advanced Antibody Detection 61 M
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5 Phenotypic Testing of Bacterial A
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5. Antimicrobial Susceptibility Tes
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Susceptibility Tests for Fastidious
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References 5. Antimicrobial Suscept
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6. Biochemical Profile-Based Microb
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7 Rapid Bacterial Characterization
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7. MALDI-TOF MS 119 photons followe
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7. MALDI-TOF MS 121 for analysis by
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7. MALDI-TOF MS 123 Sample wells Ca
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7. MALDI-TOF MS 125 also given. Thi
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7. MALDI-TOF MS 127 characteristics
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References 7. MALDI-TOF MS 129 Ande
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7. MALDI-TOF MS 131 Hindre, T., Did
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7. MALDI-TOF MS 133 von Wintzingero
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8. Probe-Based Microbial Detection
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9 Pulsed-Field Gel Electrophoresis
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9. Pulsed-Field Gel Electrophoresis
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PFGE Performance Characteristics 9.
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TABLE 10.1. Nucleic acid amplificat
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10. In Vitro Nucleic Acid Amplifica
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11. PCR and Its Variations 167 Prin
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11. PCR and Its Variations 169 Exte
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11. PCR and Its Variations 171 targ
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11. PCR and Its Variations 173 bind
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11. PCR and Its Variations 175 Reve
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11. PCR and Its Variations 177 ampl
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11. PCR and Its Variations 179 pres
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11. PCR and Its Variations 181 Soft
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11. PCR and Its Variations 183 Saik
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12. Non-PCR Amplification 185 1989;
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12. Non-PCR Amplification 187 FIGUR
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12. Non-PCR Amplification 189 This
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12. Non-PCR Amplification 191 FIGUR
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12. Non-PCR Amplification 193 and c
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12. Non-PCR Amplification 195 used
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12. Non-PCR Amplification 197 do no
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Application of the SDA Technique Re
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12. Non-PCR Amplification 201 Ancho
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12. Non-PCR Amplification 203 Baner
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12. Non-PCR Amplification 205 Guilf
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12. Non-PCR Amplification 207 Nilss
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12. Non-PCR Amplification 209 Wang,
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13. Recent Advances in Probe Amplif
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14. Signal Amplification Techniques
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TABLE 14.1. Comparison of bDNA and
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References 14. Signal Amplification
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15 Detection and Characterization o
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15. Agarose Gel Electrophoresis, So
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16. Direct Nucleotide Sequencing 26
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17. Microarrays for Microbial Chara
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18 Diagnostic Microbiology Using Re
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TABLE 18.1. Comparison of four fluo
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18. Real-Time PCR Based on FRET 295
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19. Amplification Product Inactivat
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TABLE 19.1. Comparison of inactivat
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20 Bacterial Identification Based o
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20. Analysis of 16S rRNA Gene Seque
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21 Molecular Techniques for Blood a
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Hepatitis B Surface Antigen (Anti-H
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Vironostika HIV-1 Microelisa System
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21. Blood and Blood Product Screeni
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22 Review of Molecular Techniques f
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22. Molecular Techniques for STDs D
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Traditional Diagnostic Methods 22.
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References 22. Molecular Techniques
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23 Advances in the Diagnosis of Myc
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23. Diagnosis of Mycobacterium tube
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Immunodiagnostic Methods 23. Diagno
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24 Rapid Screening and Identificati
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24. Rapid Screening and Identificat
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25 Bead-Based Flow Cytometric Assay
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A. Test 488-635 nm 680 nm Analyte o
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25. Bead-Based Flow Cytometric Assa
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26. Molecular Strain Typing 445 tha
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26. Molecular Strain Typing 447 FIG
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TABLE 26.1. Comparison of strain-ty
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26. Molecular Strain Typing 451 Som
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26. Molecular Strain Typing 453 and
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26. Molecular Strain Typing 455 pat
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Surveillance for Potential Infectio
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26. Molecular Strain Typing 459 the
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26. Molecular Strain Typing 461 of
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26. Molecular Strain Typing 463 Cow
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26. Molecular Strain Typing 465 Hea
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26. Molecular Strain Typing 467 May
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26. Molecular Strain Typing 469 Shu
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26. Molecular Strain Typing 471 Ver
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27. Molecular Differential Diagnose
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TABLE 27.11. Detection results of t
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TABLE 27.13. Detection of 25 HPV ty
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TABLE 27.16. List of pathogens iden
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TABLE 27.19. Results of the TB drug
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TABLE 27.21. Example data for the G
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28 Pathogen Detection in the Genomi
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28. Pathogen Detection in the Genom
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Cephid Smart Cycler 1600 16/run www
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28. Pathogen Detection in the Genom
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Page 1070: 526 Index B Babesia, 16S rRNA, 328
Page 1074: 528 Index Dot Blot hybridization, 3
Page 1078: 530 Index Helicobacter pylori (cont
Page 1082: 532 Index LCR. See ligase chain rea
Page 1086: 534 Index Mycobacterium tuberculosi
Page 1090: 536 Index pulsed-field gel electrop
Page 1094: 538 Index Staphylococcus aureus (MS
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Advanced Techniques in Diagnostic Microbiology

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