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Advanced Techniques in Diagnostic Microbiology

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18. Real-Time PCR Based on FRET 295<br />

Polymerization<br />

R = Reporter<br />

Q = Quencher<br />

5'<br />

3'<br />

5'<br />

Foreward<br />

Primer<br />

R<br />

Probe<br />

Q<br />

3'<br />

Reverse<br />

Primer<br />

5'<br />

3'<br />

5'<br />

5'<br />

3'<br />

5'<br />

Strand Displacement<br />

R<br />

Q<br />

3'<br />

5'<br />

3'<br />

5'<br />

5'<br />

3'<br />

5'<br />

Cleavage<br />

R<br />

Q<br />

3'<br />

5'<br />

3'<br />

5'<br />

5'<br />

3'<br />

5'<br />

Polymerization<br />

R<br />

(a)<br />

Q<br />

3'<br />

5'<br />

3'<br />

5'<br />

hv<br />

P<br />

hυ<br />

hv<br />

P<br />

hυ<br />

(b)<br />

(c)<br />

FIGURE 18.2. (a) TaqMan PCR probes. (b) FRET Probes. (c) Molecular beacons.

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