Advanced Techniques in Diagnostic Microbiology

TABLE 26.1. Comparison of strain-typing technologies.
Ease of Ease of
Technique performance a interpretation a Discrimination a Time a Reproducibility a Cost a Comercial kit
PFGE Moderate Moderate–High High High High Moderate–High No
Sequencing Low Moderate–High High Moderate High High No
RAPD High High High Low Moderate Low No
RFLP Moderate High Moderate Moderate High Low No
AFLP Moderate Moderate High Moderate Moderate Moderate–High Available b
Ribotyping Moderate Moderate Moderate Moderate High High Available c
MLST Low Moderate–High High Moderate High e High e No
Rep-PCR High High High Low High Low Available d
PFGE, pulsed-field gel electrophoresis; RAPD, randomly amplified polymorphic DNA; RFLP, restriction fragment length polymorphism; AFLP, amplified fragment
length polymorphism; MLST, multilocus sequence typing.
a Table 1 (Olive and Bean, 1999 J Clin Micribiol, 37(6):1661–1669 and/or Table 2 (Van Belkum, et al., 2001 Clin Microbilol Rev, 14(3):547–560).
b Applied Biosystems Microbial AFLP Kits.
c DuPont RiboPrinter.
d Bacterial Barcodes Diversilab System.
e Trinidade, et al. 2003 Braz J Infect Dis, 7(1):32–43.
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