Advanced Techniques in Diagnostic Microbiology

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TABLE 2.1. Comparison of 13 C-urea breath tests for H. pylori based on information at manufacturers’ Web sites. Test name FDA status Fasting Detection Sample collection time Instrument time Sensitivity a Specificity a Manufacturer BreathTek IVD b 1 h GIRMS, or 0 and 15 min Sent to specialty lab (GIRMS) 95% 95% Mereteck UBiT-IR300 5.5 min (UBiT-IR300) Helikit IVD b 4 h IRMS, or 30 min Sent to specialty lab (IRMS) 98% 95% Isodiagnostika ISOMAX 2002 not available (ISOMAX) a Based on statements in manufacturers’ product inserts. b For in vitro diagnostic use. 17
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Advanced Techniques in Diagnostic M
Page 6: Yi-Wei Tang Molecular Infectious Di
Page 10: vi Contributors Wonder Drake Depart
Page 14: viii Contributors Jacques Schrenzel
Page 18: Preface Clinical microbiologists ar
Page 22: Contents Part I Techniques 1 Automa
Page 26: Contents xv 22 Review of Molecular
Page 30: 1 Automated Blood Cultures XIANG Y.
Page 34: Interpretation of Significance 1. A
Page 38: 1. Automated Blood Cultures 7 conta
Page 42: 1. Automated Blood Cultures 9 Crump
Page 46: 2 Urea Breath Tests for Detection o
Page 50: 2. Urea Breath Tests 13 and point o
Page 54: 2. Urea Breath Tests 15 Other detec
Page 60: 18 S. Wang and X. Zheng tubes for G
Page 64: 20 S. Wang and X. Zheng Graham, D.
Page 68: 22 S. Wang and X. Zheng two test me
Page 72: 24 S. Campbell and M. L. Landry Pri
Page 76: 26 S. Campbell and M. L. Landry opp
Page 80: 28 S. Campbell and M. L. Landry dir
Page 84: TABLE 3.1. Characteristics of the t
Page 88: TABLE 3.2. Application of technique
Page 92: TABLE 3.2 (Continued) Pathogen Meth
Page 96: TABLE 3.2 (Continued) Pathogen Meth
Page 100: 38 S. Campbell and M. L. Landry Rap
Page 104: 40 S. Campbell and M. L. Landry Ger
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4 Advanced Antibody Detection YUN F
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44 Y. F. Wang grouped into several
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46 Y. F. Wang variety of substrates
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48 Y. F. Wang One reason accounting
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50 Y. F. Wang by a carbodiimide con
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52 Y. F. Wang TABLE 4.2. Type of co
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54 Y. F. Wang small-volume samples
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56 Y. F. Wang Multiplexed Bead Assa
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58 Y. F. Wang the reliability of th
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60 Y. F. Wang Hemmila, I., Dakubu,
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62 Y. F. Wang (2002). Specific, sen
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64 C. Qi, C. W. Stratton, and X. Zh
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6 Biochemical Profile-Based Microbi
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86 J. Aslanzadeh + beta hemolytic +
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88 J. Aslanzadeh Gram Negative Cocc
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90 J. Aslanzadeh Preliminary Identi
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92 J. Aslanzadeh tryptophane result
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94 J. Aslanzadeh β-naphthylamine p
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96 J. Aslanzadeh Reading the test b
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98 J. Aslanzadeh TABLE 6.1. Commonl
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100 J. Aslanzadeh Do not incubate t
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102 J. Aslanzadeh Using a sterile i
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104 J. Aslanzadeh Voges-Proskaure (
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106 J. Aslanzadeh The strip is exam
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108 J. Aslanzadeh ferrous sulfate i
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110 J. Aslanzadeh TABLE 6.2. Commer
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112 J. Aslanzadeh substrates. It is
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114 J. Aslanzadeh The reagents will
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116 J. Aslanzadeh Killian, M. (1974
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118 D. Dare Furthermore, the amount
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120 D. Dare be required. Currently,
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122 D. Dare TABLE 7.1. Comparison o
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124 D. Dare Search results Combined
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126 D. Dare (a) No. genera 4123 No.
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128 D. Dare that similarity of the
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130 D. Dare Dare, D., Sutton, H., K
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132 D. Dare McKenna, T., Lunt, M.,
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8 Probe-Based Microbial Detection a
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136 T. Hong Hybridization and Detec
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138 T. Hong Candida species can det
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140 T. Hong and 100%, respectively.
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142 T. Hong Wagner, M., Schmid, M.,
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144 F. Wu and P. Della-Latta migrat
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146 F. Wu and P. Della-Latta TABLE
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148 F. Wu and P. Della-Latta DNA d
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150 F. Wu and P. Della-Latta TABLE
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152 F. Wu and P. Della-Latta λ 1 2
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154 F. Wu and P. Della-Latta Summar
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156 F. Wu and P. Della-Latta pneumo
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10 In Vitro Nucleic Acid Amplificat
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160 H. Li and Y-W. Tang of these me
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162 H. Li and Y-W. Tang Currently,
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164 H. Li and Y-W. Tang La Rocco, M
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11 PCR and Its Variations MICHAEL L
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168 M. Loeffelholz and H. Deng to o
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170 M. Loeffelholz and H. Deng FIGU
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172 M. Loeffelholz and H. Deng be i
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174 M. Loeffelholz and H. Deng the
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176 M. Loeffelholz and H. Deng RT-P
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178 M. Loeffelholz and H. Deng Dete
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180 M. Loeffelholz and H. Deng buff
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182 M. Loeffelholz and H. Deng Grat
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12 Non-Polymerase Chain Reaction Me
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186 M.L. Pendrak and S.S. Yan TMA (
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188 M.L. Pendrak and S.S. Yan dsDNA
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190 M.L. Pendrak and S.S. Yan (van
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192 M.L. Pendrak and S.S. Yan ampli
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194 M.L. Pendrak and S.S. Yan FIGUR
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196 M.L. Pendrak and S.S. Yan RCA m
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202 M.L. Pendrak and S.S. Yan TABLE
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204 M.L. Pendrak and S.S. Yan of hu
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206 M.L. Pendrak and S.S. Yan Kwoh,
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208 M.L. Pendrak and S.S. Yan Smirn
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13 Recent Advances in Probe Amplifi
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212 D. Zhang et al. TABLE 13.1. Com
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214 D. Zhang et al. scanner after h
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216 D. Zhang et al. FIGURE 13.3. Sc
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218 D. Zhang et al. FIGURE 13.4. Sc
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220 D. Zhang et al. A. B. Invasive
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222 D. Zhang et al. FIGURE 13.6. Pr
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224 D. Zhang et al. The mecA probe
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226 D. Zhang et al. Landegren, U.,
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14 Signal Amplification Techniques:
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230 Y. F. Wang Virus (3)* (2) (4)*
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232 Y. F. Wang 1. Denaturation 2. H
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234 Y. F. Wang Application of the T
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236 Y. F. Wang specimen. A study (K
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238 Y. F. Wang amplification techno
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240 Y. F. Wang Cuzick, J., Szarewsk
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242 Y. F. Wang Qian, X., & Lloyd, R
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244 R. P. Podzorski, M. Loeffelholz
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16 Direct Nucleotide Sequencing for
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266 Tao Hong approximately 10 min (
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268 Tao Hong frequently caused prob
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270 Tao Hong for the use of the 16S
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272 Tao Hong of MRSA. The coagulase
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274 Tao Hong Gürtler, V., & Barrie
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17 Microarray-Based Microbial Ident
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278 T.J. Gentry and J. Zhou These a
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280 T.J. Gentry and J. Zhou the des
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282 T.J. Gentry and J. Zhou Communi
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284 T.J. Gentry and J. Zhou PCR-bas
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286 T.J. Gentry and J. Zhou Althoug
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288 T.J. Gentry and J. Zhou Korczak
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290 T.J. Gentry and J. Zhou Willse,
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292 X. Qin allow real-time monitori
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294 X. Qin which the observed fluor
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296 X. Qin that there would be no G
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298 X. Qin 2005). Furthermore, the
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300 X. Qin Cattoli, G., Drago, A.,
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302 X. Qin Kostrikis, L.G., Touloum
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304 X. Qin Ririe, K.M., Rasmussen,
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19 Amplification Product Inactivati
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308 S. Sefers and Y-W. Tang Most de
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310 S. Sefers and Y-W. Tang UNG Pro
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312 S. Sefers and Y-W. Tang 4' Psor
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314 S. Sefers and Y-W. Tang Isopsor
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316 S. Sefers and Y-W. Tang used is
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318 S. Sefers and Y-W. Tang Conclus
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Part II Applications
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324 X.Y. Han TABLE 20.1. The number
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326 X.Y. Han Homology Search and Re
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328 X.Y. Han TABLE 20.2. Examples o
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330 X.Y. Han Conclusion In summary,
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332 X.Y. Han Shimizu, T., Ohtani, K
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TABLE 21.1. Licensed / Approved Cli
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TABLE 21.1. (Continued) Tradename(s
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TABLE 21.1. (Continued) Tradename(s
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340 Y. Hu and I. Hirshfield gel ele
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342 Y. Hu and I. Hirshfield Since 1
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344 Y. Hu and I. Hirshfield they ar
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346 Y. Hu and I. Hirshfield Antibod
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348 Y. Hu and I. Hirshfield all ove
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350 Y. Hu and I. Hirshfield chances
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352 Y. Hu and I. Hirshfield Tang, Y
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354 A. C. T. Lo and K. M. Kam in tu
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356 A. C. T. Lo and K. M. Kam is no
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358 A. C. T. Lo and K. M. Kam stabl
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360 A. C. T. Lo and K. M. Kam Stran
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362 A. C. T. Lo and K. M. Kam organ
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364 A. C. T. Lo and K. M. Kam probe
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366 A. C. T. Lo and K. M. Kam major
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368 A. C. T. Lo and K. M. Kam Molec
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370 A. C. T. Lo and K. M. Kam a sec
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372 A. C. T. Lo and K. M. Kam cervi
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374 A. C. T. Lo and K. M. Kam E6 an
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376 A. C. T. Lo and K. M. Kam M. (2
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378 A. C. T. Lo and K. M. Kam Hippe
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380 A. C. T. Lo and K. M. Kam Larse
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382 A. C. T. Lo and K. M. Kam Nobbe
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384 A. C. T. Lo and K. M. Kam chlam
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386 A. C. T. Lo and K. M. Kam ureal
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388 A. Kilic and W. Drake Lipids wi
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390 A. Kilic and W. Drake America,
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392 A. Kilic and W. Drake MTB from
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394 A. Kilic and W. Drake with anti
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396 A. Kilic and W. Drake Molecular
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398 A. Kilic and W. Drake and monit
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400 A. Kilic and W. Drake system; t
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402 A. Kilic and W. Drake transillu
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404 A. Kilic and W. Drake Real-Time
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406 A. Kilic and W. Drake Caws, M.,
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408 A. Kilic and W. Drake Marttila,
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410 A. Kilic and W. Drake Torres, M
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412 P. Francois and J. Schrenzel an
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414 P. Francois and J. Schrenzel th
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416 P. Francois and J. Schrenzel A
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418 P. Francois and J. Schrenzel St
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420 P. Francois and J. Schrenzel Ac
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422 P. Francois and J. Schrenzel or
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424 P. Francois and J. Schrenzel Lo
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426 P. Francois and J. Schrenzel Va
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428 D. Ernst et al. several microme
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430 D. Ernst et al. reanalysis to d
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432 D. Ernst et al. Neisseria menin
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434 D. Ernst et al. A. B. SEB-beads
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436 D. Ernst et al. IL-6, IL-8, and
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pg/ml 120 100 80 60 40 20 0 120 100
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440 D. Ernst et al. Although interf
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442 D. Ernst et al. Lal, G., Balmer
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26 Molecular Strain Typing Using Re
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446 S. R. Frye and M. Healy FIGURE
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448 S. R. Frye and M. Healy Automat
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450 S. R. Frye and M. Healy TABLE 2
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452 S. R. Frye and M. Healy Noncomm
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454 S. R. Frye and M. Healy 2001).
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456 S. R. Frye and M. Healy (Stempe
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458 S. R. Frye and M. Healy isolate
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460 S. R. Frye and M. Healy molecul
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462 S. R. Frye and M. Healy Boerlin
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464 S. R. Frye and M. Healy Fey, P.
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466 S. R. Frye and M. Healy Kwara,
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468 S. R. Frye and M. Healy Pfaller
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470 S. R. Frye and M. Healy Tang, Y
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27 Molecular Differential Diagnoses
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474 J. Han The technology advanceme
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476 J. Han Targets Optimal Conditio
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478 J. Han TABLE 27.1. Comparison o
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480 J. Han TABLE 27.3. Comparison o
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482 J. Han suspension hybridization
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484 J. Han fluorescent dye is remov
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486 J. Han TABLE 27.5. List of path
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TABLE 27.8. Detection results of th
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490 J. Han common in HAI. In additi
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492 J. Han TABLE 27.12. Detection r
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494 J. Han TABLE 27.14. List of pat
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496 J. Han TABLE 27.18. Detectable
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TABLE 27.20. List of gene targets i
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500 J. Han Benefits and Impact: Del
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502 J. Han significantly increasing
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504 J. Han Hindiyeh, M., Hillyard D
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506 E. M. Marlowe and D. M. Wolk Al
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TABLE 28.1. Automated specimen proc
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510 E. M. Marlowe and D. M. Wolk Au
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512 E. M. Marlowe and D. M. Wolk La
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514 E. M. Marlowe and D. M. Wolk me
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516 E. M. Marlowe and D. M. Wolk mo
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518 E. M. Marlowe and D. M. Wolk Mo
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520 E. M. Marlowe and D. M. Wolk Ha
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522 E. M. Marlowe and D. M. Wolk Sa
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Index A acetate utilization, 100 Ac
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Index 527 rapid antigen tests, 27-2
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Index 529 flow cytometric immunoass
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Index 531 Human T-Lymphotropic Viru
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Index 533 MLEE. See multilocus enzy
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Index 535 PNA. See peptide-nucleic
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Index 537 Roseomonas, 328-329 rotav
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Index 539 T TaqMan probes, 293-296
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