Advanced Techniques in Diagnostic Microbiology

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86 J. Aslanzadeh + beta hemolytic + Bacillus sp. + Bacillus sp. − motile Gram Positive Bacilli growth in air + − Branch spore − + + − spore Modified AFB Lecithinase Actinomyces Proppionibacterium Eubacterium Lactobacillus − + − + − catalase Nocardia Rhodococcus Gordona Tsukamurella Streptomyces Actinomadura Oreskovia Rothia Reverse CAMP Dounble-zone beta hemolysis C. septicum C. difficile + − + − motile beta hemolytic H2S + in TSI Beta hemolytic on HBT Clostridium perfringens Urea − + − reverse CAMP positive Erysiplothrix rhousiopathiae Gardnerella vaginalis + − ? B. anthracis Esculin Corynebacterium sp. Brevibacterium Turicella Dermabacter Archanobacterium hemolyticum C. sordellii Spot indole + − + − Listeria monocytogenes Kurthia C. bifermentans C. barati FIGURE 6.2. Flow chart for presumptive identification of Gram positive bacilli.
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Advanced Techniques in Diagnostic M
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Yi-Wei Tang Molecular Infectious Di
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vi Contributors Wonder Drake Depart
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viii Contributors Jacques Schrenzel
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Preface Clinical microbiologists ar
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Contents Part I Techniques 1 Automa
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Contents xv 22 Review of Molecular
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1 Automated Blood Cultures XIANG Y.
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Interpretation of Significance 1. A
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1. Automated Blood Cultures 7 conta
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1. Automated Blood Cultures 9 Crump
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2 Urea Breath Tests for Detection o
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2. Urea Breath Tests 13 and point o
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2. Urea Breath Tests 15 Other detec
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TABLE 2.1. Comparison of 13 C-urea
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2. Urea Breath Tests 19 Bazzoli, F.
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2. Urea Breath Tests 21 Logan, R. (
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3 Rapid Antigen Tests SHELDON CAMPB
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3. Rapid Antigen Tests 25 antigen i
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3. Rapid Antigen Tests 27 either on
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3. Rapid Antigen Tests 29 and moves
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3. Rapid Antigen Tests 31 Applicati
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Fungi Cryptococcus Agglutination, E
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Adenovirus, enteric types 40,41 EIA
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3. Rapid Antigen Tests 37 Antigen t
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3. Rapid Antigen Tests 39 retains a
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3. Rapid Antigen Tests 41 Wilhelmi,
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4. Advanced Antibody Detection 43 D
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TABLE 4.1. Types of antibody detect
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4. Advanced Antibody Detection 47 c
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4. Advanced Antibody Detection 49 U
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4. Advanced Antibody Detection 51 a
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4. Advanced Antibody Detection 53 a
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4. Advanced Antibody Detection 55 H
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4. Advanced Antibody Detection 57 r
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4. Advanced Antibody Detection 59 A
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Page 150: 5 Phenotypic Testing of Bacterial A
Page 154: 5. Antimicrobial Susceptibility Tes
Page 166: Susceptibility Tests for Fastidious
Page 182: References 5. Antimicrobial Suscept
Page 194: 6. Biochemical Profile-Based Microb
Page 200: 88 J. Aslanzadeh Gram Negative Cocc
Page 204: 90 J. Aslanzadeh Preliminary Identi
Page 208: 92 J. Aslanzadeh tryptophane result
Page 212: 94 J. Aslanzadeh β-naphthylamine p
Page 216: 96 J. Aslanzadeh Reading the test b
Page 220: 98 J. Aslanzadeh TABLE 6.1. Commonl
Page 224: 100 J. Aslanzadeh Do not incubate t
Page 228: 102 J. Aslanzadeh Using a sterile i
Page 232: 104 J. Aslanzadeh Voges-Proskaure (
Page 236: 106 J. Aslanzadeh The strip is exam
Page 240: 108 J. Aslanzadeh ferrous sulfate i
Page 244: 110 J. Aslanzadeh TABLE 6.2. Commer
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112 J. Aslanzadeh substrates. It is
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114 J. Aslanzadeh The reagents will
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116 J. Aslanzadeh Killian, M. (1974
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118 D. Dare Furthermore, the amount
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120 D. Dare be required. Currently,
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122 D. Dare TABLE 7.1. Comparison o
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124 D. Dare Search results Combined
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126 D. Dare (a) No. genera 4123 No.
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128 D. Dare that similarity of the
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130 D. Dare Dare, D., Sutton, H., K
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132 D. Dare McKenna, T., Lunt, M.,
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8 Probe-Based Microbial Detection a
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136 T. Hong Hybridization and Detec
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138 T. Hong Candida species can det
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140 T. Hong and 100%, respectively.
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142 T. Hong Wagner, M., Schmid, M.,
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144 F. Wu and P. Della-Latta migrat
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146 F. Wu and P. Della-Latta TABLE
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148 F. Wu and P. Della-Latta DNA d
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150 F. Wu and P. Della-Latta TABLE
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152 F. Wu and P. Della-Latta λ 1 2
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154 F. Wu and P. Della-Latta Summar
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156 F. Wu and P. Della-Latta pneumo
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10 In Vitro Nucleic Acid Amplificat
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160 H. Li and Y-W. Tang of these me
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162 H. Li and Y-W. Tang Currently,
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164 H. Li and Y-W. Tang La Rocco, M
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11 PCR and Its Variations MICHAEL L
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168 M. Loeffelholz and H. Deng to o
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170 M. Loeffelholz and H. Deng FIGU
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172 M. Loeffelholz and H. Deng be i
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174 M. Loeffelholz and H. Deng the
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176 M. Loeffelholz and H. Deng RT-P
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178 M. Loeffelholz and H. Deng Dete
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180 M. Loeffelholz and H. Deng buff
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182 M. Loeffelholz and H. Deng Grat
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12 Non-Polymerase Chain Reaction Me
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186 M.L. Pendrak and S.S. Yan TMA (
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188 M.L. Pendrak and S.S. Yan dsDNA
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190 M.L. Pendrak and S.S. Yan (van
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192 M.L. Pendrak and S.S. Yan ampli
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194 M.L. Pendrak and S.S. Yan FIGUR
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196 M.L. Pendrak and S.S. Yan RCA m
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202 M.L. Pendrak and S.S. Yan TABLE
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204 M.L. Pendrak and S.S. Yan of hu
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206 M.L. Pendrak and S.S. Yan Kwoh,
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208 M.L. Pendrak and S.S. Yan Smirn
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13 Recent Advances in Probe Amplifi
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212 D. Zhang et al. TABLE 13.1. Com
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214 D. Zhang et al. scanner after h
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216 D. Zhang et al. FIGURE 13.3. Sc
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218 D. Zhang et al. FIGURE 13.4. Sc
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220 D. Zhang et al. A. B. Invasive
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222 D. Zhang et al. FIGURE 13.6. Pr
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224 D. Zhang et al. The mecA probe
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226 D. Zhang et al. Landegren, U.,
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14 Signal Amplification Techniques:
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230 Y. F. Wang Virus (3)* (2) (4)*
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232 Y. F. Wang 1. Denaturation 2. H
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234 Y. F. Wang Application of the T
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236 Y. F. Wang specimen. A study (K
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238 Y. F. Wang amplification techno
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240 Y. F. Wang Cuzick, J., Szarewsk
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242 Y. F. Wang Qian, X., & Lloyd, R
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244 R. P. Podzorski, M. Loeffelholz
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16 Direct Nucleotide Sequencing for
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266 Tao Hong approximately 10 min (
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268 Tao Hong frequently caused prob
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270 Tao Hong for the use of the 16S
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272 Tao Hong of MRSA. The coagulase
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274 Tao Hong Gürtler, V., & Barrie
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17 Microarray-Based Microbial Ident
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278 T.J. Gentry and J. Zhou These a
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280 T.J. Gentry and J. Zhou the des
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282 T.J. Gentry and J. Zhou Communi
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284 T.J. Gentry and J. Zhou PCR-bas
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286 T.J. Gentry and J. Zhou Althoug
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288 T.J. Gentry and J. Zhou Korczak
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290 T.J. Gentry and J. Zhou Willse,
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292 X. Qin allow real-time monitori
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294 X. Qin which the observed fluor
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296 X. Qin that there would be no G
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298 X. Qin 2005). Furthermore, the
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300 X. Qin Cattoli, G., Drago, A.,
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302 X. Qin Kostrikis, L.G., Touloum
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304 X. Qin Ririe, K.M., Rasmussen,
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19 Amplification Product Inactivati
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308 S. Sefers and Y-W. Tang Most de
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310 S. Sefers and Y-W. Tang UNG Pro
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312 S. Sefers and Y-W. Tang 4' Psor
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314 S. Sefers and Y-W. Tang Isopsor
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316 S. Sefers and Y-W. Tang used is
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318 S. Sefers and Y-W. Tang Conclus
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Part II Applications
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324 X.Y. Han TABLE 20.1. The number
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326 X.Y. Han Homology Search and Re
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328 X.Y. Han TABLE 20.2. Examples o
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330 X.Y. Han Conclusion In summary,
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332 X.Y. Han Shimizu, T., Ohtani, K
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TABLE 21.1. Licensed / Approved Cli
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TABLE 21.1. (Continued) Tradename(s
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TABLE 21.1. (Continued) Tradename(s
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340 Y. Hu and I. Hirshfield gel ele
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342 Y. Hu and I. Hirshfield Since 1
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344 Y. Hu and I. Hirshfield they ar
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346 Y. Hu and I. Hirshfield Antibod
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348 Y. Hu and I. Hirshfield all ove
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350 Y. Hu and I. Hirshfield chances
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352 Y. Hu and I. Hirshfield Tang, Y
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354 A. C. T. Lo and K. M. Kam in tu
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356 A. C. T. Lo and K. M. Kam is no
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358 A. C. T. Lo and K. M. Kam stabl
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360 A. C. T. Lo and K. M. Kam Stran
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362 A. C. T. Lo and K. M. Kam organ
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364 A. C. T. Lo and K. M. Kam probe
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366 A. C. T. Lo and K. M. Kam major
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368 A. C. T. Lo and K. M. Kam Molec
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370 A. C. T. Lo and K. M. Kam a sec
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372 A. C. T. Lo and K. M. Kam cervi
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374 A. C. T. Lo and K. M. Kam E6 an
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376 A. C. T. Lo and K. M. Kam M. (2
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378 A. C. T. Lo and K. M. Kam Hippe
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380 A. C. T. Lo and K. M. Kam Larse
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382 A. C. T. Lo and K. M. Kam Nobbe
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384 A. C. T. Lo and K. M. Kam chlam
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386 A. C. T. Lo and K. M. Kam ureal
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388 A. Kilic and W. Drake Lipids wi
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390 A. Kilic and W. Drake America,
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392 A. Kilic and W. Drake MTB from
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394 A. Kilic and W. Drake with anti
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396 A. Kilic and W. Drake Molecular
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398 A. Kilic and W. Drake and monit
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400 A. Kilic and W. Drake system; t
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402 A. Kilic and W. Drake transillu
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404 A. Kilic and W. Drake Real-Time
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406 A. Kilic and W. Drake Caws, M.,
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408 A. Kilic and W. Drake Marttila,
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410 A. Kilic and W. Drake Torres, M
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412 P. Francois and J. Schrenzel an
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414 P. Francois and J. Schrenzel th
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416 P. Francois and J. Schrenzel A
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418 P. Francois and J. Schrenzel St
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420 P. Francois and J. Schrenzel Ac
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422 P. Francois and J. Schrenzel or
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424 P. Francois and J. Schrenzel Lo
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426 P. Francois and J. Schrenzel Va
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428 D. Ernst et al. several microme
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430 D. Ernst et al. reanalysis to d
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432 D. Ernst et al. Neisseria menin
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434 D. Ernst et al. A. B. SEB-beads
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436 D. Ernst et al. IL-6, IL-8, and
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pg/ml 120 100 80 60 40 20 0 120 100
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440 D. Ernst et al. Although interf
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442 D. Ernst et al. Lal, G., Balmer
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26 Molecular Strain Typing Using Re
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446 S. R. Frye and M. Healy FIGURE
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448 S. R. Frye and M. Healy Automat
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450 S. R. Frye and M. Healy TABLE 2
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452 S. R. Frye and M. Healy Noncomm
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454 S. R. Frye and M. Healy 2001).
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456 S. R. Frye and M. Healy (Stempe
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458 S. R. Frye and M. Healy isolate
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460 S. R. Frye and M. Healy molecul
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462 S. R. Frye and M. Healy Boerlin
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464 S. R. Frye and M. Healy Fey, P.
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466 S. R. Frye and M. Healy Kwara,
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468 S. R. Frye and M. Healy Pfaller
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470 S. R. Frye and M. Healy Tang, Y
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27 Molecular Differential Diagnoses
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474 J. Han The technology advanceme
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476 J. Han Targets Optimal Conditio
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478 J. Han TABLE 27.1. Comparison o
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480 J. Han TABLE 27.3. Comparison o
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482 J. Han suspension hybridization
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484 J. Han fluorescent dye is remov
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486 J. Han TABLE 27.5. List of path
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TABLE 27.8. Detection results of th
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490 J. Han common in HAI. In additi
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492 J. Han TABLE 27.12. Detection r
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494 J. Han TABLE 27.14. List of pat
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496 J. Han TABLE 27.18. Detectable
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TABLE 27.20. List of gene targets i
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500 J. Han Benefits and Impact: Del
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502 J. Han significantly increasing
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504 J. Han Hindiyeh, M., Hillyard D
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506 E. M. Marlowe and D. M. Wolk Al
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TABLE 28.1. Automated specimen proc
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510 E. M. Marlowe and D. M. Wolk Au
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512 E. M. Marlowe and D. M. Wolk La
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514 E. M. Marlowe and D. M. Wolk me
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516 E. M. Marlowe and D. M. Wolk mo
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518 E. M. Marlowe and D. M. Wolk Mo
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520 E. M. Marlowe and D. M. Wolk Ha
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522 E. M. Marlowe and D. M. Wolk Sa
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Index A acetate utilization, 100 Ac
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Index 527 rapid antigen tests, 27-2
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Index 529 flow cytometric immunoass
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Index 531 Human T-Lymphotropic Viru
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Index 533 MLEE. See multilocus enzy
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Index 535 PNA. See peptide-nucleic
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Index 537 Roseomonas, 328-329 rotav
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Index 539 T TaqMan probes, 293-296
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