l - People

Select up to three hinge points using the Hinge Annotation Tool, as described above.
Click on the “Submit” button.
Orient the molecule to the desired perspective by clicking and dragging in the Jmol
window.
Click on the “color by domain” link.
The coloration of the domains goes by the following logic. All the residues prior to the
first hinge point are assigned to domain D1, all the residues between the first and second
hinge points belong to D3, all the residues between the second and third hinge points
belong to D1, and all subsequent residues belong to D3, and so on. The hinge residues
themselves belong to D2. D1 is colored orange, D2 is green, and D3 is blue.
To color by HingeMaster flexibility step 1 above is unnecessary; in step 3 click instead
“color by HingeMaster score.” In either case after a slight delay, a pop-up window will
display the generated image. A variant of this tool was used to generate some of the
images in this chapter.
Glutamine Binding Protein (GluBP) (open)
Morph ID: f927198-20246 PDB ID: 1GGG
HAG hinges (residues 89,90,178-182)
Examination of results for individual proteins can bring out salient features of the various
predictors. As a first example, we present Glutamine binding protein (GluBP). GluBP of
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