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esidues, provided an accurate measure of the degree to which a protein exhibited hinge
bending. High scores, close to unity, indicated proteins more likely to exhibit hinge
bending motion. Lower scores, below 0.9 or so, were very unlikely to do so. We sorted
the 1000 nonredundant morphs by descending normalized flexprot score (described
earlier) and annotated them in that order. Those proteins for which we could find hinges
allowing a positive answer to the question above were annotated and added to the Hinge
Atlas. Those proteins which did not exhibit hinge bending motion or for which no
suitable hinge could be found were discarded. At the end of this culling and annotation
effort, the Hinge Atlas contained 214 nonredundant annotated morphs. We also manually
annotated a small set of specifically fragment (rather than domain) hinge bending
motions which may be useful for some studies, described below.
Availability of datasets
In the course of this study we compiled a number of sets of morphs which can be viewed
on our online galleries listed and linked to on our sets page. The Hinge Atlas and
computer annotated sets are compared more rigorously in the “Statistical comparison of
datasets” section. The galleries provide easy browsing and visual inspection of morph
movies sharing certain characteristics. The sets offered include:
Nonredundant: No two morphs in this set have more than 90% sequence homology.
This set was compiled by alignment to proteins in nrdb90.
Catalytic Sites Atlas: All morphs in this set have annotated active sites which can be
highlighted in the jmol viewer.
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