ZMBH J.Bericht 2000 - Zentrum für Molekulare Biologie der ...

GPI-anchor. Moreover, in collaboration with D. Soldati
(ZMBH), we have generated several recombinant
Toxoplasma gondii strains that also expose at the
surface full size MSP-1, the p42 or the p19 processing
product, respectively. In both, the HeLa cell and
the T.gondii system, monoclonal antibodies directed
towards conformational epitopes of MSP-1 interact
efficiently with the protein at the surface of the respective
cells. Nevertheless, so far we were neither able
to unequivocally demonstrate an interaction between
MSP-1 and erythrocytes nor can we definitely rule out
such interactions. The failure to reveal the putative
affinity may be due to a number of reasons. We, therefore,
will further pursue these studies.
External Funding
During the period reported, our research was supported
by grants from the BMBF (Forschungsschwerpunkt
Tropenmedizin), the Volkswagen-Stiftung, the
Deutsche Forschungsgemeinschaft (SFB 544 “Kontrolle
tropischer Infektionskrankheiten” and Projekt-
’Sachbeihilfen’), from the EU, the BioRegion Rhein-
Neckar (BMBF - Fa. Knoll AG) and the Fonds der
Chemischen Industrie Deutschlands.
PUBLICATIONS
Tremblay, P., Meiner, Z., Galou, M., Heinrich, C.,
Petromilli, C., Lisse, T., Cayateno, J., Torchia, M.,
Mobley, W., Bujard, H., DeArmond, S.J. and Prusiner,
S.B. (1998). Doxycycline control of prion protein
transgene expression modulates prion disease in mice.
Proc. Natl. Acad. Sci. USA 95, 12580-12585.
Baron, U., Schnappinger, D., Helbl, V., Gossen, M.,
Hillen, W. and Bujard, H. (1999). Generation of conditional
mutants in higher eukaryotes by switching
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between the expression of two genes. Proc. Natl.
Acad. Sci. USA 96, 1013-1018
Freundlieb, S., Schirra-Müller, C. and Bujard, H.
(1999). A tetracycline controlled activation /repression
system for mammalian cells. J. Gene Med. 1, 4-12.
Pan, W., Ravot, E., Tolle, R., Frank, R., Mosbach, R.,
Türbachova, I. and Bujard, H. (1999). Vaccine candidate
MSP-1 from Plasmodium falciparum: a redesigned
4917 bp polynucleotide enables synthesis and
isolation of full length protein from E.coli and mammalian
cells. Nucl. Acids Res. 27, 1094-1103.
Redfern, C.H., Coward, P., Degtyarev, M.Y., Lee, E.K.,
Kwa, A., Hennighausen, L., Bujard, H., Fishman, G.I.
and Conklin, B.R. (1999). In vivo conditional expression
and signaling of a specifically designed G i -coupled
receptor. Nature Biotech. 17, 165-169.
Burghaus, P.A., Gerold, P., Pan, W., Schwarz, R.T.,
Lingelbach, K. and Bujard, H. (1999). Analysis of
recombinant merozoite surface protein-1 of Plasmodium
falciparum expressed in mammalian cells. Mol.
Biochem. Parasitol. 104, 171-183.
Lavon, I., Goldberg, I., Amit, S., Landsman, L., Jung,
S., Tsuberi, B., Barshack, I., Kopolovic, J., Galun, E.,
Bujard, H. and Ben-Neriah, Y. (2000) High susceptibility
to bacterial infection, but no liver dysfunction,
in mice compromised for hepatocyte NF-κB activation.
Nat. Med. 6, 573-577.
Baron, U. and Bujard, H. (2000) The Tet repressor
based system for regulated gene expression in eukaryotic
cells: principles and advances. Methods Enzymol.
327, 659-686.
Mansuy, I. and Bujard, H. (2000) Tetracycline-regulated
gene expression in the brain. Curr. Op. Neurobiol.,
in press.
Urlinger, S., Baron, U., Thellmann, M., Hasan, M.,
Bujard, H. and Hillen, W. (2000). Exploring the
sequence space for tetracycline dependent transcriptional
activators: novel mutations yield expanded
range and sensitivity. Proc. Natl. Acad. Sci. USA, 97,
7963-68.
THESES
Diploma
Epp, Christian (1998): Analyse der humoralen Immunantwort
von Aotus-Affen gegen eine Immunisie–
rung mit MSP-1 aus Plasmodium falciparum
Schönig, Kai (1998): Konstruktion und Analyse von
„integrierten Regulationseinheiten“ zur Tetrazyklin
kontrollierten Genexpression in Säugerzellen
Schmid, Christina (1999): Untersuchungen zum Oberflächenprotein
1 von Merozoiten (MSP-1) des Mala–
riaerregers Plasmodium falciparum: Kontrollierte Synthese
des 42kDa-Fragments in Säugerzellen
Dissertation
Baron, Udo (1998): Weiterentwicklung der Methodik
der Tetrazyklin-kontrollierten Genexpression zur Ana–
lyse der Funktion von Genen in komplexen eukaryotischen
Systemen.
Awards
Ruperto Carola Preis 1998 der Universität Heidelberg,
for outstanding PhD Thesis to Udo Baron
STRUCTURE OF THE GROUP
E-mail: bujardh@sun0.urz.uni-heidelberg.de
Group leader Bujard, Hermann, Prof. Dr.
Guest scientist del Portillo, Hernando, Prof. Dr.*
Research
Associate Freundlieb, Sabine, Dr.
Postdoctoral Baron, Udo, PhD
fellows Fernandez-Becerra, Carmen, PhD*
Hasan, Mazahir, PhD*
Löw, Rainer, PhD*
Weerasuriya, Siromi, PhD*
Ph.D. students Berger, Stefan, Dipl.Chem.
Epp, Christian, Dipl.Biol.
Idler, Irina, Dipl.Biol.*
Kolster, Kathrin, Dipl.Biol.*
Schönig, Kai, Dipl.Biol.
Türbachova, Ivana, Dipl.Biol.
Westerfeld, Nicole, Dipl.Biol.
Diploma
students Baldinger, Tina*
Schmid, Christina*
Techn. assistants Rittirsch, Melanie*
Schirra-Müller, Christiane
Wahedy, Soraya*
* part of the time reported
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