Self-Assembly of Synthetic and Biological Polymeric Systems of ...

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temperatures, and solvent polarity. IX. Ethanol regulated the hydrogen bonding extension, the attractive hydrophobic interactions, and the protein accessible surface area which, in turn, give rise to intermediate structural states prone to form aggregate. X. The HSA aggregation rate at physiological condition is faster than under acidic one owing to the pre-existing structural differences of the protein molecules in solution and to changes in the nature and strength of intermolecular interaction upon incubation. XI. Ethanol promoted an α-helix to β-sheet conformational transition at intermediate alcohol concentrations, whereas at low and high ethanol contents α-helix prevailed as the predominant structure at room temperature and physiological pH. XII. Electrostatic and hydrophobic interactions are important to control stacking of β- sheets favouring the formation of amyloid-like fibrils at physiological pH and high temperature. 6.8.- Relevant aspects on the gold and magnetic nanowires I. We report a method to obtain metallic Au nanowires by using Lys protein fibrils as bioscaffolds. The metallic coverage of nanofibrils could be easily controlled by changing the numbers of sequential additions of gold growth solution into the fibril solution overall already preformed Au nanocrystals. In this way, a continuous coverage of the fibril surface is obtained as a result of the growth of pre-existing nanoparticles and generation of new ones on the fibrils surface and subsequent fusion of adjacent as-synthesized nanoparticles. II. UV-Vis spectra results showed that the hybrid fibrils can successfully catalyze the reduction reaction from p-nitrophenol to p-aminophenol by NaBH4. Moreover, the fibril metallic fibrils provide larger reaction rates values 1.5-4 than other hybrid materials reported, previously, as for example glucose-reduced Au nanoparticle networks, PAMAM-dendrimer-supported spherical nanoparticles amongst others. III. On the other hand, we report a method for obtaining magnetic Fe3O4 nanowires based on the use of HSA and Lys protein fibrils as bioscaffolds for generation of a complete magnetic coating layer on the biotemplate surface by controlled Fe nanoprecipitation in situ in the presence of NH4OH. IV. Thanks to their large saturation magnetization, the 1D magnetic nanowires can serve 216
as efficient MRI contrast agents, with transverse relaxivities larger than those obtained previously for other 1D nanostructures derived from different methods. Their capability as contrast agents was verified by obtaining MRI images at different Fe concentrations in phantoms. 217
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Grupo de Física de Coloides y Pol
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Agradecimientos A mi Familia A mi P
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v List of papers I. Self-assembly p
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2.3 2.4 2.5 2.6 2.7 2.8 2.9 2.10 2.
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5.4 5.5 5.3.2 5.5.1 Chapter 6 Kinet
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amiloides de la proteína albúmina
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vesiculares son el resultado de la
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origina debido a condiciones ambien
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con las fibras vecinas más cercana
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Abstract In the present work, we in
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[Au]/[protein] molar ratio and numb
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synthetic polymers are obtained fro
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On the other hand, in terms of chem
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Additionally, to obtain a better kn
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therefore, characteristic of solid
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presence of electrostatic charge in
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Contents 2.1 2.2 2.3 2.4 2.5 2.6 2.
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where w is the meniscus’ weight d
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that they exert little force one on
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This oscillating dipole acts as an
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(APD) and its associated optics (pi
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where r is the distance of the dipo
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When , the former equation can be s
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autocorrelation function (ACF). In
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and, therefore, . The autocorrelati
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A transmission electron microscope
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2.5.- Atomic force microscopy (AFM)
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ecause of the energy loss in the ex
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Figure 2.15. Schematic representati
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ackbone of proteins. Since proteins
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According to classical mechanics, t
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chemical composition, configuration
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2.9.1.- Viscoelasticity Many materi
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where is the total strain rate, whi
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Using equations 2.40 and 2.41 in eq
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scattering process that incoming X-
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maximum intensity of the peak, Imax
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translation of the reciprocal latti
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For a single crystal, the chance to
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excitation; namely, a valance elect
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Figure 2.27. Distinction between si
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microscope, there are two polarized
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In particular, we use SQUID to meas
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are aligned by means of an external
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on the digital profile of a drop im
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Laser confocal microscopy. Confocal
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Fluorescence spectroscopy. Fluoresc
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Superconducting quantum interferenc
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temperatures, the chains are mixed
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e detected by a given method. Therm
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subsequently, of their thermodynami
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a) O b) H 2C CH 2 O H 2C CH Figure
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Contents 4.1 4.2 4.3 4.3.1 CHAPTER
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7108 Langmuir, Vol. 24, No. 14, 200
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7110 Langmuir, Vol. 24, No. 14, 200
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7112 Langmuir, Vol. 24, No. 14, 200
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7114 Langmuir, Vol. 24, No. 14, 200
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7116 Langmuir, Vol. 24, No. 14, 200
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15704 J. Phys. Chem. C, Vol. 114, N
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4.3.1.- Relevant aspects I. For E12
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Contents 5.1 5.2 5.3 5.4 5.5 5.1.1
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acids whose lateral side chains cha
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adjacent segments of an antiparalle
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5.2.1.- Unfolded state Over the las
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5.2.4.- Energy landscape: protein a
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molecular medicine viewpoint, prote
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shows the typical nucleation-depend
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the energy landscape of the aggrega
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exposed loop region. The secondary
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Biophysical Journal Volume 96 March
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Fibrillation Pathway of HSA 2355 q
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Fibrillation Pathway of HSA 2357 mo
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Fibrillation Pathway of HSA 2359 in
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Fibrillation Pathway of HSA 2361 Fu
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Fibrillation Pathway of HSA 2363 FI
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Fibrillation Pathway of HSA 2365 Wh
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Fibrillation Pathway of HSA 2367 of
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Fibrillation Pathway of HSA 2369 17
Page 179 and 180: Influence of Electrostatic Interact
Page 181 and 182: Fibrillation Process of Human Serum
Page 187: Fibrillation Process of Human Serum
Page 190 and 191: 12392 J. Phys. Chem. B, Vol. 113, N
Page 199 and 200: 5 10 15 20 25 30 35 40 45 Soft Matt
Page 201 and 202: 5 10 15 20 25 r h,app = kT/(6πηD
Page 203 and 204: 5 10 15 20 25 30 35 40 45 50 below,
Page 205 and 206: 5 10 15 20 25 30 55 Figure 3: Selec
Page 207 and 208: 10 15 Soft Matter Cite this: DOI: 1
Page 209 and 210: 5 10 15 Soft Matter Cite this: DOI:
Page 211: 5 65. L. A. Sikkink, M. Ramirez-Alv
Page 214 and 215: ions would interact electrostatical
Page 216: pubs.acs.org/JPCL Figure 3. (a) Tim
Page 220 and 221: (54) Almeida, N. L.; Oliveira, C. L
Page 222 and 223: netospirillum magneticum strain AMB
Page 224 and 225: One-Dimensional Magnetic Nanowires
Page 233 and 234: Conclusions The work has two parts:
Page 235 and 236: equire a highly organized and unsta
Page 237 and 238: References 1. Hiemenz, Paul C. Poly
Page 239 and 240: 33. Wang, Z. L. HANDBOOK OF MICROSC
Page 241 and 242: 67. Polymers Get Organized. Bucknal
Page 243 and 244: 94. Are there Pathways for Protein
Page 245 and 246: 123. Designing conditions for in vi
Page 247: 151. SERS-based diagnosis and biode
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