Self-Assembly of Synthetic and Biological Polymeric Systems of ...

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Laser light scattering. Dynamic and static light scattering (DLS and (SLS) intensities were measured by means of an ALV-5000F (ALV-GmbH) instrument with vertically polarized incident light of wavelength λ= 488 nm supplied by a CW diode pumped Nd;YAG solid-state laser supplied by Coherent, Inc. and operated at 2 W. Polarized optical microscopy. POM experiments were made by using a Zeiss Axioplan optical microscope (Carl Zeiss Ltd., Welwyn Garden City, U.K.) A total magnification of either 50x or 100 X was used. A polarizer and analyzer were put in fixed position, orthogonal to one another for the cross-polarizer imaging. Images were taken using a Kodak digital camera mounted on the top of the microscope. 84
Laser confocal microscopy. Confocal microscopy was performed on a Leica TCS SP2 confocal system mounted on a Leica DM-IRE2 upright microscope, using a 40X objective. For fluorescence measurements, Thioflavin T was added to spherulitic samples and excited using the 458 nm line of an argon ion laser. Simultaneously, transmission images were obtained with crossed polarizers in place using the 633 nm line of a He-Ne laser. Rheometry. The rheological properties of the samples were determined using a Bohlin CS10 rheometer with water bath temperature control. Coutte geometry (bob, 24.5 mm in diameter, 27 mm in height, cup, 26.5 in diameter, 29 mm in height) was used for fluid samples. Samples of very high modulus were investigated using cone and plate geometry (diameter 40 mm, angle 4 0 ). Frequency scans of storage (G’) and lost (G’’) moduli were recorded for selected copolymer concentrations and temperatures with the instrument in oscillatory shear mode and with the strain amplitude (A) maintained at a low value (A < 0.5 %) by means of auto- stress facility of the Bohlin software. This ensured that measurements of G’ and G’’ were in the linear viscoelastic region. 85
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Grupo de Física de Coloides y Pol
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Agradecimientos A mi Familia A mi P
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v List of papers I. Self-assembly p
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2.3 2.4 2.5 2.6 2.7 2.8 2.9 2.10 2.
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5.4 5.5 5.3.2 5.5.1 Chapter 6 Kinet
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amiloides de la proteína albúmina
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vesiculares son el resultado de la
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origina debido a condiciones ambien
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con las fibras vecinas más cercana
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Abstract In the present work, we in
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[Au]/[protein] molar ratio and numb
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synthetic polymers are obtained fro
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On the other hand, in terms of chem
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Additionally, to obtain a better kn
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therefore, characteristic of solid
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presence of electrostatic charge in
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Contents 2.1 2.2 2.3 2.4 2.5 2.6 2.
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where w is the meniscus’ weight d
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that they exert little force one on
Page 47 and 48: This oscillating dipole acts as an
Page 49 and 50: (APD) and its associated optics (pi
Page 51 and 52: where r is the distance of the dipo
Page 53 and 54: When , the former equation can be s
Page 55 and 56: autocorrelation function (ACF). In
Page 57 and 58: and, therefore, . The autocorrelati
Page 59 and 60: A transmission electron microscope
Page 61 and 62: 2.5.- Atomic force microscopy (AFM)
Page 63 and 64: ecause of the energy loss in the ex
Page 65 and 66: Figure 2.15. Schematic representati
Page 67 and 68: ackbone of proteins. Since proteins
Page 69 and 70: According to classical mechanics, t
Page 71 and 72: chemical composition, configuration
Page 73 and 74: 2.9.1.- Viscoelasticity Many materi
Page 75 and 76: where is the total strain rate, whi
Page 77 and 78: Using equations 2.40 and 2.41 in eq
Page 79 and 80: scattering process that incoming X-
Page 81 and 82: maximum intensity of the peak, Imax
Page 83 and 84: translation of the reciprocal latti
Page 85 and 86: For a single crystal, the chance to
Page 87 and 88: excitation; namely, a valance elect
Page 89 and 90: Figure 2.27. Distinction between si
Page 91 and 92: microscope, there are two polarized
Page 93 and 94: In particular, we use SQUID to meas
Page 95 and 96: are aligned by means of an external
Page 97: on the digital profile of a drop im
Page 101 and 102: Fluorescence spectroscopy. Fluoresc
Page 103: Superconducting quantum interferenc
Page 106 and 107: temperatures, the chains are mixed
Page 108 and 109: e detected by a given method. Therm
Page 110 and 111: subsequently, of their thermodynami
Page 112 and 113: a) O b) H 2C CH 2 O H 2C CH Figure
Page 115: Contents 4.1 4.2 4.3 4.3.1 CHAPTER
Page 118 and 119: 7108 Langmuir, Vol. 24, No. 14, 200
Page 128 and 129: 15704 J. Phys. Chem. C, Vol. 114, N
Page 138 and 139: 4.3.1.- Relevant aspects I. For E12
Page 141 and 142: Contents 5.1 5.2 5.3 5.4 5.5 5.1.1
Page 143 and 144: acids whose lateral side chains cha
Page 145 and 146: adjacent segments of an antiparalle
Page 147 and 148: 5.2.1.- Unfolded state Over the las
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5.2.4.- Energy landscape: protein a
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molecular medicine viewpoint, prote
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shows the typical nucleation-depend
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the energy landscape of the aggrega
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exposed loop region. The secondary
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Biophysical Journal Volume 96 March
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Fibrillation Pathway of HSA 2355 q
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Fibrillation Pathway of HSA 2357 mo
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Fibrillation Pathway of HSA 2359 in
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Fibrillation Pathway of HSA 2361 Fu
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Fibrillation Pathway of HSA 2363 FI
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Fibrillation Pathway of HSA 2365 Wh
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Fibrillation Pathway of HSA 2367 of
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Fibrillation Pathway of HSA 2369 17
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Influence of Electrostatic Interact
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Fibrillation Process of Human Serum
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Page 185 and 186:
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Page 190 and 191:
12392 J. Phys. Chem. B, Vol. 113, N
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Page 194 and 195:
Page 196 and 197:
Page 199 and 200:
5 10 15 20 25 30 35 40 45 Soft Matt
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5 10 15 20 25 r h,app = kT/(6πηD
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5 10 15 20 25 30 35 40 45 50 below,
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5 10 15 20 25 30 55 Figure 3: Selec
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10 15 Soft Matter Cite this: DOI: 1
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5 10 15 Soft Matter Cite this: DOI:
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5 65. L. A. Sikkink, M. Ramirez-Alv
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ions would interact electrostatical
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pubs.acs.org/JPCL Figure 3. (a) Tim
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(54) Almeida, N. L.; Oliveira, C. L
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netospirillum magneticum strain AMB
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One-Dimensional Magnetic Nanowires
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temperatures, and solvent polarity.
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Conclusions The work has two parts:
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equire a highly organized and unsta
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References 1. Hiemenz, Paul C. Poly
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33. Wang, Z. L. HANDBOOK OF MICROSC
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67. Polymers Get Organized. Bucknal
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94. Are there Pathways for Protein
Page 245 and 246:
123. Designing conditions for in vi
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151. SERS-based diagnosis and biode
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