Barbieri Thesis - BioMedical Materials program (BMM)

More documents

Recommendations

Info

Chapter 5 – Alkali surface treatment effects mineralization process has been suggested to occur in living organisms. [378] Thus, alkali surface treatment improved the surface mineralising potential of the composites, which decreases in presence of proteins. Significant more serum proteins adsorbed onto the treated composites as compared to the non–treated one. The better performances in protein adsorption may be assigned to the larger exposure of apatite particles and/or to the improved surface nano–texture of the surface–etched materials. However, despite its larger nano– roughness M2 adsorbed less serum proteins than M1. This may be connected to the larger surface hydrophilicity of M2 compared to M1. It has been suggested that the formation of a layer of water on hydrophilic surfaces generates an energy barrier for the adsorption of most proteins [379, 380, 381] because they need to change their conformation to reach an energetic equilibrium with the surface. However, some proteins such as albumin are reported to be conformationally ‘flexible’ protein able to adsorb also on hydrophilic surfaces. [382] As discussed earlier, surface mineralization occurred in SBF/FBS solution generating apatite nano–textured surfaces which offered more adsorption sites to proteins for their adsorption. At the same time surfaces in contact with serum alone did not mineralize and thus had less adsorption sites available (i.e. only the apatite initially exposed on their surfaces). Consequently, surface mineralization significantly increased the adsorption of serum proteins and albumin in all the considered materials. This may be related to the apatite based chemistry and nano–structure of the precipitated layers.This fact is strengthened by the reporting that proteins, such as fibronectin and vitronectin, adsorb in larger amounts onto rougher surfaces, [383, 384] and they are calcium–affine. [153, 250, 251, 346] 5.5. Conclusion Summarizing, the roughest material (i.e. M2) adsorbed proteins, generated a mineralised surface and was able to trigger osteogenic differentiation. In view of these properties we expected it would have at least initiated bone formation but it did not. This study showed that surface roughness is a parameter that should be kept in mind when designing biomaterials as it affects the final material properties and can have implications on its biological performances, both in vitro and in vivo. However, it is necessary to carefully consider the fact that in vitro biological systems (e.g. cell culture, protein adsorption) used to study the biomaterial may lead far from the real in vivo performance of the material. 116
The results described in this Chapter will be submitted for publication. CHAPTER 6 Fluid uptake as instructive factor in biomaterials for bone tissue regeneration
Page 3 and 4:
INSTRUCTIVE COMPOSITES FOR BONE REG
Page 5 and 6:
INSTRUCTIVE COMPOSITES FOR BONE REG
Page 7:
献给潘臻 A mamma e papà A Stef
Page 11 and 12:
Summary Summary Developing new biom
Page 13:
Summary containing 96%mol. L-lactid
Page 16 and 17:
Samenvatting De behoefte aan een de
Page 18 and 19:
Samenvatting structuur, bespoedigt
Page 20 and 21:
Riassunto velocità di dissoluzione
Page 22 and 23:
Riassunto generale, ha migliorato i
Page 25 and 26:
Chapter 1 - Introduction 1.1. Eukar
Page 27 and 28:
Chap pter 1 - Introducction Interst
Page 29 and 30:
Chapter 1 - Introduction bone marro
Page 31 and 32:
Chap pter 1 - Introducction Figure
Page 33 and 34:
Chapter 1 - Introduction regenerati
Page 35 and 36:
Chapter 1 - Introduction Key concep
Page 37 and 38:
Chapter 1 - Introduction grown in c
Page 39 and 40:
Chapter 1 - Introduction Mainly, th
Page 41 and 42:
Chapter 1 - Introduction All these
Page 43 and 44:
Chap pter 1 - Introducction materia
Page 45 and 46:
Chapter 1 - Introduction protein, m
Page 47:
CHAPTER 2 The role of gels in bone
Page 50 and 51:
Chapter 2 - The role of gels in ins
Page 52 and 53:
Page 54 and 55:
CChapter 2 - The role r of gels in
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 69:
CHAPTER 3 Instructive composites: e
Page 72 and 73:
Chapter 3 - Instructive composites:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Chapter 3 - Instructive compos site
Page 80 and 81:
Page 82 and 83:
CChapter 3 - Instructive composit t
Page 84 and 85:
Page 86 and 87:
Page 88 and 89: Chapter 3 - Instructive composites:
Page 91: CHAPTER 4 Controlling dynamic mecha
Page 94 and 95: Chapter 4 - Conntrol of mechanical
Page 96 and 97: Chapter 4 - Control of mechanical a
Page 111: CHAPTER 5 Effects of alkali surface
Page 114 and 115: Chapter 5 - Alkali surface treatmen
Page 124 and 125: Chapter 5 - Al lkali surface treaat
Page 141 and 142: Chapter 6 - Fluid uptake as instruc
Page 151 and 152: Chapter 6 - Flu uid uptake as insst
Page 157 and 158: Figure 7. Mass left (a) annd fluid
Page 169: Chapter 6 - Flu uid uptake as insst
Page 173 and 174: Chapter 7 - Polymer molecular weigh
Page 179 and 180: ūapatite = 100 · mdry · pap Chap
Page 185 and 186: Chapter 7 - Polymer mole ecular wei
Page 187 and 188: Chapter 7 - Polymer mole ecular wei
Page 189 and 190:
Chapter 7 - Polymer mole ecular wei
Page 191 and 192:
Page 193 and 194:
Chapter 7 - Polymer molecular weigh
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203:
CHAPTER 8 General discussion
Page 206 and 207:
Chapter 8 - General discussion can
Page 208 and 209:
Chapter 8 - General discussion cons
Page 210 and 211:
Chapter 8 - General discussion 8.1.
Page 212 and 213:
Chapter 8 - General discussion rese
Page 215 and 216:
References [1] Waggoner B. Eukaryot
Page 217 and 218:
References induction of rank in ost
Page 219 and 220:
References [72] Kokovay E, Wang Y,
Page 221 and 222:
References [102] McBeath R, Pirone
Page 223 and 224:
References molecule-mediated TGF- t
Page 225 and 226:
References [159] Autumn K, Sitti M,
Page 227 and 228:
References [189] Teixeira AI, McKie
Page 229 and 230:
References [215] Tang L, Jennings T
Page 231 and 232:
References [242] Wei G, Ma PX. Stru
Page 233 and 234:
References [270] Atkinson BL, Hanks
Page 235 and 236:
References [297] Sato I, Akizuki T,
Page 237 and 238:
References hematopoietic stem cell
Page 239 and 240:
References [352] Cullinane DM, Sali
Page 241 and 242:
References [382] Norde W, Giacomell
Page 243:
References differentiation of rat B
Page 246 and 247:
Acknowledgments thank you for all t
Page 248 and 249:
Acknowledgments desidero tutto il m
Page 250 and 251:
List of publications List of public
show all

Barbieri Thesis - BioMedical Materials program (BMM)

Create successful ePaper yourself

Delete template?

Save as template?