Barbieri Thesis - BioMedical Materials program (BMM)

Chapter 7 – Polymer mole ecular weight annd
instructive co omposites
(0 out of 6) ). Mature bonee
formed tightly
to the mineralised
surfaces
of ML gran nules, while
non–minerralised
bone mmatrix,
with active a osteobllast–like
cells s, was observ ved on the
outer surfaace
of the matture
bone (Fig gure 7). Thesee
results indic cated that the process of
bone formaation
was still active after 12
weeks of immplantation.
Th he fact that mature
bone
grew tightlyy
onto the minneralised
apat tite layers wass
confirmed by y BSEM image es taken in
the same sspots
used forr
histology (Fig gure 8a,c).
Figure 7. Hisstology
images ( (non–decalcified, , 1% methylene blue and 0.3% basic fuchsin sta aining) of ML
explant harveested
from back muscle of dogs after 12 weeks. . Image (b) is th he magnification of the box in
image (a). Heeterotopic
bone formation was in nduced and an aactive,
but not yet
mineralized, bo one formation
zone (long black
arrows) is vvisible.
Osteoblas sts (long white aarrows)
are embe edded in bone matrix
and are
synthesising new bone. The inner part of the
bone is an immmature
woven and a mineralized tissue (white
stars). Surfacce
mineralisationn
occurred (blac ck triangles) andd
bone tightly for rmed onto these e mineralized
layers. Soft tiissues
(ST) and bbulk
of the mater rial (ML) are indiccated
as well. The
box in (b) indic cates the spot
where BSEMM
images have beeen
taken (Figure e 8c).
As comparred
to the starrting
materials s, thicker apat
both compposites
could bbe
observed (Figure ( 8). Th
may have precipitated from body flu uids and nuc
significant increase in thhickness
(p