Barbieri Thesis - BioMedical Materials program (BMM)

Chapter 6 – Flu uid uptake as insstructive
factor
Figure 13. Microscopy detaails
of (a) MLD, (b) ( MLDL and (cc)
MDL explants stained s with meth hylene blue
and basic fuuchsin.
Green triangles
indicate surface s mineralizzation
layers onto o the granules. Black
arrows
indicate acttive
osteoblasts on immature bon ne matrix and whhite
arrows show w osteocytes. Sta ars indicate
ongoing bone
matrix formatiion,
letters B and d ST stands for mmature
bone and soft s tissue respec ctively.
6.4.1. Composites
The commposites
had similar surfac ce mineralizin
simulatedd
body fluid aall
granules were w covered
soaking inn
foetal bovine
serum (FBS S) solution for
and MDLL
had comparable
fluid abs sorption. Intere
adsorbedd
similar amouunts
of serum proteins (Figu
up less fluids
and adsoorbed
less pro oteins (Figure
MLD, thee
higher fluid uuptake
in MLD DL and MDL a
surfaces and FBS, inccreasing
the probability p of
short afteer
implantatioon
in vivo, biological b mol
clotting faactors,
could have largely y adsorbed on
allowing aadhesion
of innflammation
ce ells. [216]
ng potential, as a within two o days in
by mineralize ed apatite layers.
After
a week at bo ody temperature,
MLDL
estingly, these e two compos sites also
ure 6). In turn, MLD significa antly took
6). In this situ uation, as com mpared to
allowed better r contact betw ween their
serum protein n adsorption. Similarly,
ecules from body fluids, including
n the surface es of MLDL and a MDL
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