Barbieri Thesis - BioMedical Materials program (BMM)

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Chapter 6 – Flu uid uptake as insstructive factor Figure 12. Low magnification scans of (a) MLD, M (b) MLDL aand (c) MDL explants stained with h methylene blue and baasic fuchsin. Arroows indicate bone e (dark red) wherreas granules hav ve colors varying g from white to pink. Soft tissues are colored with violet/blue. v Blackk squares indica ate the spots where w high magnificatioon images were ttaken with light microscopy m (Figurre 13). 6.4. Disccussion The resullts of this studdy showed tha at fluid uptake in biomateria als can be controlled by means off polymer monnomer chemis stry in the casee of composite materials, or o through the surfacce grain size (thus the surfa ace area) in cceramics. Those materials that t could absorb mmore fluids alsoo showed high her degradabiility and protein adsorption potential. Besides this, they couuld induce he eterotopic bonne formation. However, th he results obtained in one of the two classes of o biomaterial ccould not be fully f extrapolat ted to the other onee due to multifaactorial issues s. 138
Chapter 6 – Flu uid uptake as insstructive factor Figure 13. Microscopy detaails of (a) MLD, (b) ( MLDL and (cc) MDL explants stained s with meth hylene blue and basic fuuchsin. Green triangles indicate surface s mineralizzation layers onto o the granules. Black arrows indicate acttive osteoblasts on immature bon ne matrix and whhite arrows show w osteocytes. Sta ars indicate ongoing bone matrix formatiion, letters B and d ST stands for mmature bone and soft s tissue respec ctively. 6.4.1. Composites The commposites had similar surfac ce mineralizin simulatedd body fluid aall granules were w covered soaking inn foetal bovine serum (FBS S) solution for and MDLL had comparable fluid abs sorption. Intere adsorbedd similar amouunts of serum proteins (Figu up less fluids and adsoorbed less pro oteins (Figure MLD, thee higher fluid uuptake in MLD DL and MDL a surfaces and FBS, inccreasing the probability p of short afteer implantatioon in vivo, biological b mol clotting faactors, could have largely y adsorbed on allowing aadhesion of innflammation ce ells. [216] ng potential, as a within two o days in by mineralize ed apatite layers. After a week at bo ody temperature, MLDL estingly, these e two compos sites also ure 6). In turn, MLD significa antly took 6). In this situ uation, as com mpared to allowed better r contact betw ween their serum protein n adsorption. Similarly, ecules from body fluids, including n the surface es of MLDL and a MDL 139
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INSTRUCTIVE COMPOSITES FOR BONE REG
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INSTRUCTIVE COMPOSITES FOR BONE REG
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献给潘臻 A mamma e papà A Stef
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Summary Summary Developing new biom
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Summary containing 96%mol. L-lactid
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Samenvatting De behoefte aan een de
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Samenvatting structuur, bespoedigt
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Riassunto velocità di dissoluzione
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Riassunto generale, ha migliorato i
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Chapter 1 - Introduction 1.1. Eukar
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Chap pter 1 - Introducction Interst
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Chapter 1 - Introduction bone marro
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Chap pter 1 - Introducction Figure
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Chapter 1 - Introduction regenerati
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Chapter 1 - Introduction Key concep
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Chapter 1 - Introduction grown in c
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Chapter 1 - Introduction Mainly, th
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Chapter 1 - Introduction All these
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Chap pter 1 - Introducction materia
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Chapter 1 - Introduction protein, m
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CHAPTER 2 The role of gels in bone
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Chapter 2 - The role of gels in ins
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CChapter 2 - The role r of gels in
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CHAPTER 3 Instructive composites: e
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Chapter 3 - Instructive composites:
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Chapter 3 - Instructive compos site
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CChapter 3 - Instructive composit t
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CHAPTER 4 Controlling dynamic mecha
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Chapter 4 - Conntrol of mechanical
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Chapter 4 - Control of mechanical a
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Page 111: CHAPTER 5 Effects of alkali surface
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Page 157 and 158: Figure 7. Mass left (a) annd fluid
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Chapter 8 - General discussion 8.1.
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Chapter 8 - General discussion rese
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References [1] Waggoner B. Eukaryot
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References induction of rank in ost
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References [72] Kokovay E, Wang Y,
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References [102] McBeath R, Pirone
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References molecule-mediated TGF- t
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References [159] Autumn K, Sitti M,
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References [189] Teixeira AI, McKie
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References [215] Tang L, Jennings T
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References [242] Wei G, Ma PX. Stru
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References [270] Atkinson BL, Hanks
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References [297] Sato I, Akizuki T,
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References hematopoietic stem cell
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References [352] Cullinane DM, Sali
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References [382] Norde W, Giacomell
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References differentiation of rat B
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Acknowledgments thank you for all t
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Acknowledgments desidero tutto il m
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Barbieri Thesis - BioMedical Materials program (BMM)

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