Barbieri Thesis - BioMedical Materials program (BMM)
Barbieri Thesis - BioMedical Materials program (BMM)
Barbieri Thesis - BioMedical Materials program (BMM)
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Chapter 7 – Polymer mole ecular weight annd<br />
instructive co omposites<br />
Figure 15. RRecorded<br />
(a) storrage<br />
modulus and<br />
(b) loss tangennt<br />
of M38 and M60. M The measur rements were<br />
performed onn<br />
dry samples in ddry<br />
air at 37ºC.<br />
7a.3.5. Aniimal<br />
study<br />
Five samples<br />
of each coomposite<br />
were e intramusculaarly<br />
implanted d in five sheep p, and after<br />
six monthss<br />
all samples were retrieved.<br />
The explannts<br />
were surro ounded by thin<br />
layers of<br />
connectivee<br />
tissue and mmuscle.<br />
Histological<br />
observvations<br />
demon nstrated that heterotopic h<br />
bone formaation<br />
was trigggered<br />
in all M38 M implants bbut<br />
in none of f M60 samples s (Table 8,<br />
Figure 16). . Mature bonee<br />
formed tightly<br />
to the minerralised<br />
surface es of ML granules,<br />
while<br />
non–minerralised<br />
bone mmatrix,<br />
with active a osteobllast–like<br />
cells s, was observ ved on the<br />
outer surfaace<br />
of the mmature<br />
bone indicating onngoing<br />
bone formation (F Figure 17).<br />
Amongst thhe<br />
granules, bbone<br />
marrow has h been observed<br />
indicating<br />
the bone remodelling<br />
may have ooccurred<br />
overr<br />
six months.<br />
Table 8. Bone<br />
incidence, histoomorphometry<br />
an nd fluorescent reesults<br />
after 6–month<br />
implantation in sheep.<br />
M38 M60<br />
Bone incidence<br />
5/5 0/5<br />
Bone in available<br />
space [%] 7.8±2.3 0±0<br />
Figure 16. Low<br />
magnificationn<br />
scans of explan nts. (a) Heterotoppic<br />
bone formed in all M38 implan nts. (b) None<br />
of the M60 coomposites<br />
induceed<br />
bone formation n. Bone is indicatted<br />
by the dark pink p areas.<br />
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