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Improving the identification, handling and storage of “difficult” seeds ...

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2.7. LESOTHO presentation<br />

Lesotho National Plant Genetic Resources Centre<br />

Mr Sebili Naha<br />

Supporting On-Farm management & improvement <strong>of</strong> PGRFA (question 2.1)<br />

The Lesotho National Plant Genetic Resources Centre (LesNPGRC) works in collaboration with a<br />

local NGO, World Vision Lesotho, to conserve plant genetic resources on-farm. LesNPGRC<br />

distributes germplasm to farmer associations who are affiliated with World Vision Lesotho.<br />

Participating farmers are in <strong>the</strong> lowl<strong>and</strong>s <strong>and</strong> <strong>the</strong> foothills, mainly in <strong>the</strong> nor<strong>the</strong>rn (Butha-Bu<strong>the</strong>) <strong>and</strong><br />

central (Nazareth) areas. Germplasm distributed includes both Zea mays <strong>and</strong> Phaseolus sp.<br />

Individual farmers coming to request <strong>seeds</strong> are also informed about on-farm conservation <strong>and</strong><br />

eventually asked to participate in <strong>the</strong> activities.<br />

Germplasm <strong>storage</strong> facilities (question 5.3)<br />

Germplasm is stored as active collections in cold <strong>storage</strong> at LesNPGRC, <strong>and</strong> duplicate base<br />

collections at SADC Plant Genetic Resources Center (SPGRC). The general objective <strong>of</strong> <strong>the</strong>se<br />

activities is to ensure medium- to long-term conservation <strong>of</strong> both indigenous <strong>and</strong> exotic genetic<br />

material <strong>of</strong> socio-economic importance <strong>and</strong> to promote <strong>the</strong>ir utilization for crop improvement <strong>and</strong><br />

development <strong>of</strong> sustainable agriculture. In terms <strong>of</strong> facilities, LesNPGRC has a small <strong>storage</strong> room<br />

with only six freezers. We are currently faced with a serious problem <strong>of</strong> limited space to<br />

accommodate additional freezers, coupled with frequent power failure.<br />

Drying facilities/methods<br />

LesNPGRC has one dryer used for drying seed. This is not currently functioning, so <strong>the</strong>re is a lot <strong>of</strong><br />

material waiting to be dried. Sun drying is used only to lower <strong>the</strong> moisture content if collected or<br />

harvested material shows high moisture.<br />

Measuring seed moisture status<br />

Seed moisture status is measured after reception <strong>of</strong> germplasm <strong>and</strong> during drying. When germplasm<br />

has reached 5% moisture content, it is <strong>the</strong>n packaged for <strong>storage</strong>. A few <strong>seeds</strong> are ground using a<br />

seed grinder <strong>and</strong> placed in a moisture analyzer from which a moisture content reading is taken. This<br />

method however wastes many <strong>seeds</strong>.<br />

Storage containers<br />

LesNPGRC uses aluminum foil bags, which are put in cartons <strong>and</strong> placed in freezers.<br />

Viability monitoring (question 5.5)<br />

The seed genebank consists <strong>of</strong> living materials which must be monitored frequently for viability.<br />

We are compelled because <strong>of</strong> frequent power cuts to regularly test our material for viability. This is<br />

carried out by <strong>the</strong> Seed Testing Section in <strong>the</strong> Agronomy Division. Regeneration <strong>of</strong> <strong>the</strong> material is<br />

conducted if viability is below 85%.<br />

Constraints (question 5.10)<br />

▪ LesNPGRC is faced with staff shortages. Current staff are also expected to do o<strong>the</strong>r duties <strong>of</strong><br />

<strong>the</strong> department. The staff at <strong>the</strong> center <strong>the</strong>refore do not work full-time.<br />

▪ LesNPGRC faces a problem <strong>of</strong> regular power cuts, which may affect <strong>the</strong> material in <strong>storage</strong>.<br />

Sometimes this happens during weekends, meaning that even if power returns, freezers will be<br />

<strong>of</strong>f for a number <strong>of</strong> days.<br />

▪ The small <strong>storage</strong> room needs expansion to safely store <strong>the</strong> material.

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