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Improving the identification, handling and storage of “difficult” seeds ...

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2.15. SUDAN presentation<br />

Sudan Plant Genetic Resources Unit<br />

Mr. Awadelkarim Alamelhuda Ahmed<br />

Supporting On-Farm management & improvement <strong>of</strong> PGRFA (question 2.1)<br />

The program participates with communities in <strong>the</strong> conservation <strong>of</strong> plant genetic resources through<br />

ex-situ <strong>and</strong> in-situ conservation. The conservation <strong>of</strong> farmer varieties by in-situ methods<br />

conservation are encouraged by <strong>the</strong> program <strong>and</strong> at <strong>the</strong> same time <strong>the</strong> seed <strong>and</strong> field banks are used<br />

to maintain <strong>the</strong> genetic variability. The program also redistributes <strong>seeds</strong> to regions which loose local<br />

germplasm because <strong>of</strong> displacement or food shortages.<br />

Germplasm <strong>storage</strong> facilities (question 5.3)<br />

In <strong>the</strong> PGR program, <strong>the</strong> following equipment is available:<br />

▪ 19 deep freeze chests, 10 in good working order<br />

The germplasm is stored using <strong>the</strong> following methods:<br />

▪ Seed gene bank - more than 8000 accessions from different crops are maintained in deep<br />

freezers in long tem <strong>storage</strong> (-20ºC <strong>and</strong> 5-7% mc), with space for medium- <strong>and</strong> short-term<br />

<strong>storage</strong>.<br />

▪ Field gene bank: This in Kassala area for <strong>the</strong> conservation <strong>of</strong> Musa sp.; more than 400<br />

accessions were conserved in a field in <strong>the</strong> river Gash.<br />

Drying facilities/methods<br />

▪ 1 seed drying machine<br />

▪ 6 split unit air-conditioners, 4 in good working order<br />

▪ 5 window air conditioners, 3 in good working order<br />

The drying <strong>of</strong> <strong>seeds</strong> in <strong>the</strong> gene bank is done immediately after seed collection <strong>and</strong> seed<br />

multiplication through shed drying, dehumidification <strong>and</strong> silica gel drying. The optimum moisture<br />

content varies with <strong>the</strong> crop <strong>and</strong> <strong>the</strong> type <strong>of</strong> conservation which could be long-term or medium-term<br />

or short-term.<br />

Measuring seed moisture status<br />

▪ 1 oven<br />

▪ 2 precision top balances, 1 in good working order<br />

▪ seed moisture analyser<br />

The seed moisture is measured for any accession before conservation after seed recipient or seed<br />

multiplication <strong>and</strong> <strong>the</strong> oven method is used to determine <strong>the</strong> moisture content<br />

Storage containers<br />

▪ 3 sealing machines for aluminium envelopes, 2 in good working order<br />

The <strong>seeds</strong> collected <strong>and</strong> obtained from <strong>the</strong> multiplication process are first kept in bags. When ready<br />

for conservation, <strong>the</strong>y are put into <strong>the</strong> aluminium foil envelopes. O<strong>the</strong>r containers used for large<br />

quantities include cotton sacks <strong>and</strong> plastic or iron barrels. Smaller quantities are put into poly<strong>the</strong>ne<br />

bags <strong>and</strong> glass containers.<br />

Viability monitoring (question 5.5)<br />

Viability monitoring is supposed to be practiced every 5 years. This done through testing <strong>the</strong> seed<br />

by <strong>the</strong> seed germination method. However at present, viability is only tested on seed samples <strong>of</strong>

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