Table Of Contents

Click the “Calculate” button to calculate the results. The simulation window will update
with a rough graph, and the “Results” column at the right of the screen will have values for the
cell and syringe concentrations. The calculated C value is listed below; its background is colorcoded.
This value is equal to 5 in “Minimum Protein” mode, 100 in “Highest Quality” mode,
and will vary if the user chooses to change cell concentration manually. The c-value predicts the
shape or sigmoidicity of the curve. Optimal values for C are between 5 and 500 (green); values
between 1 and 5, and 500 and 1000 should work but may not give the best result (yellow). C
values less than 1 or greater than 1000 will probably not yield usable data (red). The user may
adjust the two experimental concentrations by using the change buttons beside each
concentration box. Once the cell concentration has been changed, clicking “Calculate” again
will update the suggested syringe concentration relative to the new cell concentration.
Any warnings will appear in the status bar near the top of the screen. These include
“High heat warning”, meaning that the estimated heat that will be generated is more than the
instrument can detect.
It is highly recommended that the users look carefully at the projected curve and make
sure that the shape and rough values are reasonable before proceeding.
A pair of options at the bottom of this tab allows users to work in K d or K a , and to choose
whether to view the simulation plot using raw heat per injection (DH) or the heat normalized to
the molar ratio (NDH).
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