Table Of Contents

Turn on the ThermoVac vacuum pump. The vacuum will pull the detergent solution
from the beaker, through the cell and into the waste flask. Do not allow the waste flask to
become full enough for liquid to be sucked into the ThermoVac’s vacuum pump, as this may
cause damage.
Once sufficient detergent solution has passed through the cell, remove the plastic tubing
from the solution and placed into another beaker containing 100 ml or more of water for rinsing.
After rinsing with water, remove the plastic tubing from the rinse water and allow time for the
vacuum to drain the fluid out of the plastic tubing, then remove the cleaning apparatus from the
cell and remove the remaining water from the cell using a long needle syringe.
If this cleaning method is insufficient, fill the cell with ~5% LiquiNox and set the
instrument to thermostat at 70°C for several hours. Be careful to cool the cells before removing
the LiquiNox, as hot liquid may shatter the loading syringe.
Syringe Cleaning:
Conceptually, cleaning the syringe is similar to using the ThermoVac to clean the sample
cell; the vacuum pulls liquid from a beaker, through the syringe, and into the waste beaker in the
ThermoVac chamber. With the syringe in the filling hole, attach the filling tubing. You will
need to line up the hole in the pipette with the hole at the top of the syringe. Spin the syringe
assembly to find the hole, and then turn it so that the hole is turned “out”, toward the outer filling
port. The screw on the end of the tubing will align itself as it is screwed in (see image below).
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