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REVIEW QUESTIONS AND EXERCISES 585
25. The objective of a study by Abrahamsson et al. (A-22) was to investigate the anti-thrombotic effects
of an inhibitor of the plasminogen activator inhibitor-1 (PAI-1) in rats given endotoxin. Experimental
subjects were male Sprague–Dawley rats weighing between 300 and 400 grams. Among
the data collected were the following measurements on PAI-1 activity and the lung 125 I-concentration
in anesthetized rats given three drugs:
Plasma PAI-1 Activity
125 I-Fibrin in the Lungs
Drugs (U/ml) (% of Ref. Sample)
Endotoxin 127 158
175 154
161 118
137 77
219 172
260 277
203 216
195 169
414 272
244 192
Endotoxin + PRAP-1 low dose 107 49
103 28
248 187
164 109
176 96
230 126
184 148
276 17
201 97
158 86
Endotoxin + PRAP-1 high dose 132 86
130 24
75 17
140 41
166 114
194 110
121 26
111 53
208 71
211 90
Source: Dr. Tommy Abrahamsson. Used with permission.
26. Pearse and Sylvester (A-23) conducted a study to determine the separate contributions of
ischemia and extracorporeal perfusion to vascular injury occurring in isolated sheep lungs and
to determine the oxygen dependence of this injury. Lungs were subjected to ischemia alone,
extracorporeal perfusion alone, and both ischemia and extracorporeal perfusion. Among the data
collected were the following observations on change in pulmonary arterial pressure (mm Hg)
and pulmonary vascular permeability assessed by estimation of the reflection coefficient for
albumin in perfused lungs with and without preceding ischemia: