Trimellitate Category; Revised Summaries - US Environmental ...

IIRAn ENV/JMfiXW(99)13
GENETIC TOXICITY 1N VITRO (NON-BACTERTAL IN VITRO TEST)
TEX SUBSTANCE
. Idenlity: Tris~2-ethythexyl]benzene-l,2,4-~~~~~~~la~e
. Remark: Sourct: Daihachi Kagaku Kogyo Co., Lid. Lol. Nn, N-60601
Purity: >99.0% Kept a1 room iemperatuse in a dark glacc until use
METHOD
. Methnd: Guideline for Screening Toxicity Te&ng aK.&emicals [Japan)
. Test type: ChromosornaI aberration test
1 C&P: YeS
c Year: 1996
c SpeckdStr4in: c)IldIu cell
c Metabolic activation: with and withour S9 from rat liver, induced wittphenobarbital md
$6benzoflavone,
l Staiistical methods Fisher’s exact analysis
. Study Dtsign:
RESULTS
1 Genotoxic elTlxts:
For contjnuous ueatmenl, cells were treated for 24 or 48 hrs wi,irhout $9.
For short-term treatment, cells were treated for 6 hrs with and without SS
and cultivated with fresh media for 18 hrs.
Cunce~tionr: 49 (continuous treatmat): 0,1.3,2.5, 5.0 m@-nL
-S9 (short-term treatment): 0,13,2.5, 5.0 m@m.L
+S9 (short-term treatment): 0, 1.3, 2.5,5.0 rngknl
Pim?shsrz 2
Solvent: Acetone
P&tire calrtrols: Mitomycin C for oonlinuous treatrment
Cyclnphasphamide for short-term treaYnent
Toxicity was not observed up to50 mglrnl in continuous and short-1erm treatment with or
without S9 mix.
Clastogeniciry polyploidy
With merabolic activation: I+1 A 1x1 [+I A [xl
Witiout metabolic activation: [ ] [ 1 [ x ] [ ] [ ] [ x ]
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