Trimellitate Category; Revised Summaries - US Environmental ...

TOXlClTY l-0 AQUATIC PILANTS (E.G., ALGAE)
TEST SUBSTANCE
. Identity: Tris(2-ethvlhex~l)benzene-1,24-t~~r~~yla~e
II Rrmarks: Source: Tokyo Kasei Kogyo Ox, Ltd. Lot NO, AX01
Purity: >95.rS%
MrnOD
. Mclhod~guidelinefa~l~wed : OECD TG 201
. Test lype : Static.
I GLP : Yes
. Year : 1998
l Species/strain # and smm%:Se!enas#um cqmimnu#m ATCC22662 Ipurehascd lrom ATCC)
. Elemcnl basis: Area under the growth curve.
I Exposure period: 72 h.
. Analytical mc&orin& Yes, measured by HPLC at start and end of tie test (72hr).
c Slalisticd methods: Bartlett lest for homogeneiiy in variances and One-wamova (ECOTOX-
Statistics Ver.1.0 beta-edition R1.4) were used for EC&, IL&, and NOEC
determination @=O.OS>.
Remarks field br TwlCwwlitiww :
Test organisms Laboratory culture: OECL? medium
Method of cultivation: Shaking ar 1OOrpm
Controls: DECD medium. ECM of potassium dichromate was 0.41 m&.
Test Condltins Test temperature range: 2322 “C
Grawth/tesc medium; OECD medium.
Shaking: 1OCl qxn
Dilution water source: OECD medium.
Exposure vessel type: 100 ml, OECD medium in a 300 mL Erlcnmeyer
flask with a silicon cap which alIcw~ ventilaticm.
Water chemistry in test @H) in nt least one replicate of each concentration
(at swt and end of thr: test): pH=7.37+4 at start and 8.3-U at md of the
test (72. h).
Stock solutibns preparation: No stock solution was prepared. Test
&emical was diluted lolOOmg& {st3ubilizer, HCQ-4-O 100m&L)with
OECD medium and stesiliscd with fil tu before USC.
Light levels and quality during exposure: 4,7564,822 lax, continuous
illuminalicm.
Tesl design Number af replicates: Triplicate
ConcezHradons: 0, 100 m&IL and dispersant control were &led.
Initial cell number in celis/mL: 1x104
Method of calcularing mtxrt measured cancentsati~nsGeamttric mean.
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