Trimellitate Category; Revised Summaries - US Environmental ...

DRAFT ENV/JM/EXCH(99)23
REsuLTs
Nominalccrncenlrations:
0, 100 (mg/L) and dispersanl control.
Meosurcd amcentretions :
At star1 of the kst {O hr), cl.O,80.6, d.O(mg/L)
At end of Ihe tesl(72 hr), d.C(Ij8.7, x1.0 (mglL)
Unil : me
Results: (calculated based on nominal concentrations)
(1) Growth inhibition (conrparisan of BPCB under growth curve)
E& (O-72 II) > 1lXl m&I
NOEC (O-72 b)> ZCQ mg/L
(Z} Growth inhibition (comparison of growth rates}
E&(24-48)> 1oomg/L
EC,(?4-72)s 1WmgL
NOEC (24-72) > lf3l mg,L
Was con td response sil tishctory:
Yes: Mean cell density increased to 270~10~ cells/mL (270~fold increase) after
72 hr for cantrol. Mean cell density increased to 215x10” ~el’ls/m.~ (275
fold increase) afkr 72 hr for Dispersant CCTIU~L
Statistical results zbs apptyriate:
Significant difference in the growth CUIX was not observed between rqlues at
100 mg.& and in each conrrol.
Remarks field for Results:
- Biological observations
Cell density at each flask at each measuring point:
Nominal Concentration (m@L) CXI Derrsily (x10’ cellslmL)
Ohr 24hr 4Shr 72hr
conCro1 1.oM.w 6.s*so 50.5s 3.48 270.5+2%0
Dspetsant Cm t ml l.Om33 9.3k1.66 57.5% 9.39 275.2+1-?.22
1 WD L.cl~O.oo l&1+7.82 65.1f12.82 2x3.3-F 7.98
(Each value represents the mean of three sample counfs.)
Growth curves: Logarithmic growth until end of the test (72 h).
Percent biomass/grau?h rate inhibition per concentration: NOI described.
Ohscrvarions: Test group(lHImg/L) showed normal and similargmwrh ta that of c.onVtll (Z33 fold
increase afkx 72 hs).
CONCLUSJONS
(1)Growth inhibilioecomperison of area under growth curve) EC, (O-72 h) > 100 rngfli.
NOEC (O-72 h) 3 100 m&k
(2)Growth inhibition{comprison of gruw~h rates) EC, {24-48) r 100 mgjL
EC& (B-72) > 100 mg,2
NOEC (24-72) 3 100 mgt’L
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