Trimellitate Category; Revised Summaries - US Environmental ...

IJRAF-I’. Eh’V/JMfiXCH(99)13
C.HItONlC l”OXICJTY TO AQUTIC INVER’I’El3lL4TES (c.g., DMH,VZA) (2)
TEST SbJ’BSTANCE
1 Identity: Tris(2-ethylhexyl)benzenrc1,~9-uicarb~xyla1e
l ncmarks: Source: Tokyo Kasei Kogyo Co., Ltd. Lot No. AX01
Purity: >95.0%
METHOD
* NelhmI; OECD TG 211 (revised edition of No.202).
. Test type; Semi-static.
. GLP: Yes
. Tear: 1998
. Analyticid procedures: Yes. Measured by HPLC 2-3 times a week (before and aficrlhs:
replacement of the lcsl water3
+ Spe&dStrnin: Dapkniu mugnu
. Test details: Semi-slotic (water renewal; 3 times a week), open-system+
+ Stalisticd methods: Eco-Statics (Version 1.0 beta-edition R1.4)
Remarks field for Test Cmditions :
Test organisms: Source, supplier, any pre-trealment, breeding method: Supplied by NIES
(Japan).
Age at study initiation: Juveniles within 24h old.
Control group: Yes.
Test conditions Stock soluli~ns preparaiicm and stability: No solvent used. Test chemica1 ’
was dguted to 1.Ovvt.B [with solubilizer HCO-40 l.thvk% contrts1Ied)
with diluting water @endt M4) before use. Solubilincr conccn~ration was
controlled lODmg,& with working solution (HCO-40 l.Owt.%}.
Test temperature range: 19.9-2CU3 “C (avenge temperature 2O*c).
Exposure vessel type: 80mL lest sAlion
beakers per treatment
in a 100 JIIL, glass beaker; 10
Dilution water SOUTCC: Elendt M4(DECD guideline No,21 1 Anna
Dilutioln water chemistry: Hardness: 251mg/L, as CaC.0,
2)
Lighting: ~1. ,200 IX, 16h:8h light-darkness cycle
Waler chemislry in test: DO= 7.0-9.2mgL; pH=7.4-7.9.
Feeding: Chl~re&a rqr.&ris, O-1-0.2 mgCIday1individual
Eicrnent {unit) basis: Mean cumulative numbers of juveniles produced per adult (reproducrion)
Test design: Number of replicates=lO; individuals per replicate-lo;
Concentrations: Q 55.6, and 100 mg/L, because 48h-IX& for parent
Daphnia (Aculc immobilization
was also tested.
test) wes>I80mg&. Dispersant control
Method of calculating mean measured concentrations.Gwme~ic mean.
Exposure period: 21 d
Analytical monitoring: By KPLC analysis. 99.7-101.3% af the nominal concentration at
preparation; 94,7-99.3% just before the renewal of the test waux (tiler 2
days expsure).
33
I. ).,_.I ,,I .,I. “., “l,^,l_. “,” .._,.,-- “.“. -“+,-- ..,, ..~~----~-.m”-“----__ ..-- I*_ ,” _.-_-.,, ” _ . . ..__* ,_(_, -...-,