Trimellitate Category; Revised Summaries - US Environmental ...

TEST SUBSTANCE
1 Identity:
Remarks:
;;IETwOD
. Mrlhod:
. Test type:
. GLP;
* Year:
l Analytical proccdnrcs:
. SpecWSLrain:
. Test details:
l Slalistiwl mclhocb:
Tris(2-elhylhexyl)bennzene-l,~4-tricarboxylase
Source: Nuoplaz 6965
ASTM and USEPA
Row-throqh condition
Yes
1984
Yes. Measured by GLC, on 0,4,‘7.14,21day)
Daphnia mapa
Dynamic flow-through
&NOVA, 2WANOV& arcsin transfOrmacion and Fisher’s protected
Least Significant Differemx (LSD)
Remarks Geld for TestConditions :
Test organisms: Source; in house culture
Age at study iniriation: Juveniles within 24h old,
Control group: Yes (control and solvent control)
Test conditions Dilution Solvent for tincentrated stock standards : Acetone (1.049mglmL)
A propcllnional diluter system was used for the intermitten introduction of test
material and dilution water irrtcl the test chambers.
Test temperature range: 18-22 “C (average tenqxxattwe 20°C).
Well water was delivered 10 the chambers as a minimum rate of Z.DrnI+Q~ljn.
Exposure vcsscl type: YOOmL test sdution in a 1003 mL glass beaker; 4
beakers per trat.ment
Dilution water chemistry: Hardness and ot.hcr chat-act&tics arc rqmrtcd.
Dilulion WateJ pH in test: pH=8.3-8.4.
Lighting: 37-74 foo,tcandIts, 16h:$h light-darkness cycle
Feeding: Algae (Selenastrum capricornulum) three times B day
Supplemented with B trout chow suspension at icast twice a week
ElemeM (unit) basis: Mean cumulative numbers of juvennes produced per ad& (reprotiuction)
Growth {length} of parental Daphnia
brig-term suTviva1
Test design: Number of replicate&; individuals per rcplicatc=~U:
Method of calculating mean measured Concent~tionsGeom~~rjc mean.
Exposure period: 21 d
Analytical monimring: By GLC analysis. 3%101% of the nominal cantinlration atPreparira!iUr.
RESULTS
. Numinal crDnccntrations: 0, 0.0074, 0.012, 0.027,0,048, 0.100 mg/L
1 Mcssurcd concetllrations:
Measured cwwen!ption ol lest chemical during Zl-day exposure
Nctn~irwl tonceniretinn Measured mnccn~rahm (day, mg/L)
bnd1,) 0 4 7 14 21 mean
30