In situ and Ex situ Conservation of Commercial Tropical Trees - ITTO

312Analysis of microsatellite DNA diversity in D. aromaticaSamples of 30 leaves from each population were collected randomly. PCRwas performed using the seven primer pairs (Shc02, Shc03, Shc04, Shc07,Shc09, Shc11 and Shc17) that showed specific amplification in D. aromatica.Allele sizes were estimated for each locus and scored. The program BIOSYS-1 (Swofford and Selander 1989) was used to test for deviation from HardyWeinberg equilibrium, and to calculate allele frequencies, mean sample size perlocus, mean number of alleles per locus, observed and expected heterozygosities,fixation index, genetic differentiation (G ST) and genetic distance (Nei 1978).Effective number of alleles per locus (A e) was calculated according to Crowand Kimura (1970). A statistic derived for microsatellite DNA loci, R ST(Goodman 1997), was used to estimate levels of genetic differentiation andgene flow between populations. As R STis based on a stepwise mutation model,those loci that do not follow this model, namely Shc07 and Shc11, were excludedfrom this estimation. A jackknife procedure (without Kanching) was made todetermine the effect the west coast population had on the overall diversity.ResultsAllele frequencyAllele frequencies for the seven loci are presented in Table 1. The most frequentallele at each locus differed from population to population. Some alleles werepresent only in certain populations.