In situ and Ex situ Conservation of Commercial Tropical Trees - ITTO

374Table 3. Test of homogeneity of the pollen pool over all the maternal trees.Locus NGpi 302Pgm 317Me 308Ugp 310AAllele frequencies G st DF χ 2 (a)fam1 fam2 fam3 Fam4 fam5 fam6 fam7 fam81 0.34 0.73 0.05 0.45 0.04 0.55 0.03 0.362 0.15 0.06 0.84 0.07 0.87 0.35 0.28 0.543 0.46 0.15 0.08 0.45 0.01 0.05 0.03 0.054 0.05 0.06 0.03 0.04 0.08 0.05 0.66 0.05 0.318 7 288.22 *1 0.66 0.03 0.74 0.86 0.98 0.14 0.83 0.782 0.24 0.81 0.15 0.07 0.01 0.76 0.12 0.173 0.05 0.09 0.08 0.03 0.00 0.05 0.02 0.024 0.05 0.03 0.03 0.03 0.00 0.05 0.02 0.02 0.313 7 297.74 *1 0.42 0.77 0.62 0.18 0.22 0.26 0.50 0.752 0.44 0.12 0.23 0.65 0.72 0.52 0.17 0.173 0.05 0.05 0.03 0.10 0.03 0.11 0.02 0.024 0.05 0.03 0.10 0.03 0.02 0.05 0.02 0.025 0.05 0.03 0.03 0.03 0.02 0.05 0.02 0.02 0.538 7 662.61 *1 0.05 0.07 0.03 0.10 0.01 0.27 0.10 0.052 0.23 0.19 0.50 0.27 0.01 0.09 0.03 0.053 0.19 0.37 0.08 0.49 0.63 0.39 0.10 0.354 0.24 0.03 0.26 0.03 0.34 0.04 0.52 0.255 0.19 0.03 0.05 0.03 0.00 0.13 0.13 0.256 0.05 0.27 0.05 0.03 0.00 0.04 0.10 0.027 0.05 0.03 0.03 0.03 0.00 0.04 0.03 0.02 0.127 7 236.02 *(a)χ 2 = 2NG st(A - 1), where N = number of seeds examined, G st= genetic differences betweenpollen and ovule pool, A = number of alleles and (A-1) = degree of freedom (DF).*Significance levels are p < 0.05.Single locus estimates of outcrossing value ranged from 0.212 to 0.968,with an unweighted mean (average single locus outcrossing rate) of 0.630 (Table1). Estimation of population outcrossing rates suggested that there was asubstantial level of inbreeding. This was further supported by the inbreedingcoefficient based on the assumption of inbreeding equilibrium, which wasestimated as 0.149 (Table 1). Inferences about inbreeding other than selfing(i.e., biparental mating) can be made from the comparison between multilocusand average single locus outcrossing rates. Comparison of these two values inTable 1 showed that the multilocus outcrossing estimated was significantly higherthan the average single-locus estimates (t m- t s= 0.111 ± 0.026, p < 0.05). Thismay indicate that the plantation was established using closely related seedsources.