In situ and Ex situ Conservation of Commercial Tropical Trees - ITTO

505screening. Finally, 16 primers with high reproducibility and clear bands werechosen for the population study.Data analysisPopulation analysisRAPD bands were scored as present (1) or absent (0), and a (1/0) data matrixwas constructed. Frequency of alleles was calculated by the square rootmethod. Proportion of genetic diversity residing among populations (Gst) wascalculated according to Hartl and Clark (1989), and genetic diversity (He)according to Nei and Li (1979).Individual analysisThe UPGMA cluster analysis was carried out using PHYLIP ver. 3.57c andgenetic distance (D) was calculated according to Nei and Li (1979).ResultsSeventy polymorphic RAPD bands were obtained from 13 primers. The geneticparameters calculated from the RAPD data are summarized in Table 2.Heterozygosity (H e) within population was high (0.310) in Jambi population(Sumatra) but low in both of the Kalimantan populations (0.257 and 0.247 inSungai Mahakam and Tanah Grogot populations, respectively). The values withinsubpopulations were lower than that within the Jambi population but higherthan those within the Kalimantan populations. The G STvalue was 0.152 among3 populations. The values between the two regions (Sumatra and Kalimantan),between populations in the same region (Sungai Mahakam and Tanah Grogotpopulations, Kalimantan region), and between subpopulations (Dalek andSadarnila subpopulation in Jambi population, Sumatra) were 0.088, 0.080, and0.065 respectively.Genetic distances between individuals (d) were also calculated. Themean value within populations was 0.341; lower than the mean of betweenpopulations (0.393), and between regions the mean value was the highest (0.406),as expected. However, the mean value within subpopulations (0.355) washigher than that of between subpopulations (0.353) or within population (0.341).The UPGMA dendrogram of each individual is shown in Fig. 1. Although thedivergence was not clear, two major clusters were observed. Cluster 1 consistedof only the individuals in Kalimantan region, but cluster 2 consisted of both theKalimantan and Sumatra regions.