Aging Aging

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Assessing Antioxidant Status 139addition of ascorbate oxidase should not result in any difference in the absorbancegiven as there is no ascorbic acid to destroy in these solutions.4. Guidelines on quality control samples and expected performance:For ease of use and reliability, aqueous ascorbic acid solutions at 100, 250,500, and 1000 µM (equivalent to 200, 500, 1000, and 2000 µM FRAP, preparedfresh daily) and aged QC serum freshly spiked with ascorbic acid are recommendedas quality control samples. These should be run in parallel with testsamples to actively monitor the performance of the test and to ensure comparabilitywith previous results.Expected precision and sensitivity of the FRASC assay:Precision is high in FRASC: typical within- and between- run CVs obtained inour laboratories are, respectively,
140 Benzie and Strain2. Benzie, I. F. F. (1996) Lipid peroxidation: a review of causes, consequences, measurementand dietary influences. Int. J. Food Sci. Nutr. 47, 233–262.3. Maxwell, S. R. J. and Lip, G. Y. H. (1997) Free radicals and antioxidants in cardiovasculardisease. Br. J. Clin. Pharmacol. 44, 307–317.4. Emerit, I. (1994) Reactive oxygen species, chromosome mutation and cancer: possiblerole of clastogenic factors in carcinogenesis. Free Radic. Biol. Med. 16, 99–109.5. Yu, B. P. (1996) Aging and oxidative stress: modulation by dietary restriction. FreeRadic. Biol. Med. 21, 651–668.6. Harman, D. (1996) Aging and disease: extending functional lifespan. Ann. NY Acad.Sci. 786, 321–336.7. Harman, D. (1956) Aging: a theory based on free radical and radiation chemistry.J. Gerontol. 2, 298–300.8. Beckman, K. B. and Ames, B. N. (1998) The free radical theory of aging matures.Physiol. Rev. 78, 547–581.9. Gariballa, S. E. and Sinclair, A. J. (1998) Nutrition, ageing and ill health. Br. J. Nutr.80, 7–23.10. Loft, S. and Poulson, H. E. (1996) Cancer risk and oxidative DNA damage in man.J. Mol. Med. 74, 297–312.11. Duthie, S. J., Ma, A., Ross, M. A., and Collins, A. R. (1996) Antioxidant supplementationdecreases oxidative damage in human lymphocytes. Cancer Res. 56,1291–1295.12. Heitzer, T., Just, H., and Munzel, T. (1996) Antioxidant vitamin C improves endothelialdysfunction in chronic smokers. Circulation 94, 6–9.13. Levine, G. N., Frei, B., Koulouris, S. N., Gerhard, M. D., Keaney, J. F., and Vita,J. A. (1996) Ascorbic acid reverses endothelial vasomotor dysfunction in patientswith coronary artery disease. Circulation 93, 1107–1113.14. Ness, A. R., Chee, D., and Elliot, P. (1997) Vitamin C and blood pressure—anoverview. J. Hum. Hyperten. 11, 343–350.15. Diplock, A. T. (1994) Antioxidants and disease prevention. Mol. Aspects Med. 15,293–376.16. Frei, B., Keaney, J. F., Retsky, K. L., and Chen, K. (1996) Vitamin C and E and LDLoxidation. Vitam. Horm. 54, 1–34.17. Samman, S., Brown, A. J., Beltran, C., and Singh, S. (1997) The effect of ascorbicacid on plasma lipids and oxidisability of LDL in male smokers. Eur. J. Clin. Nutr.51, 472–477.18. Sweetman, S. F., Strain, J. J., and McKelvey, V. J. (1997) Effect of antioxidantvitamin supplementation on DNA damage and repair in human lymphoblastoidcell. Nutr. Cancer 27, 122–130.19. Wen, Y., Cooke, T., and Feely, J. (1997) The effect of pharmacological supplementationwith vitamin C on low-density lipoprotein oxidation. Br. J. Clin. Pharmacol.44, 94–99.20. Diplock, A. T., Charleux, J. L., Crozer-Willi, G., Kok, F. J., Rice-Evans, C.,Roberfroid, M., Stahl, W. and Vina-Ribes, J. (1998) Functional food science anddefence against reactive oxidative species. Br. J. Nutr. 80, S77–S112.
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Understanding Aging 9the intestine
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Understanding Aging 117. Do long-li
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Understanding Aging 13is, the great
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Understanding Aging 15While this re
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Understanding Aging 172. Pearl, R.
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Understanding Aging 1945. Perovic,
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24 Cristofalo, Volker, and Allenmen
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26 Cristofalo, Volker, and Allenrec
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34 Cristofalo, Volker, and AllenNa
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40 Cristofalo, Volker, and Allenb.
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46 Cristofalo, Volker, and Allen33.
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48 Cristofalo, Volker, and Allen61.
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50 Cristofalo, Volker, and Allenhum
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52 Cristofalo, Volker, and Allen125
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54 Pawelecdiluted cells on an irrad
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56 Pawelec3. Method3.1. Source of C
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58 Pawelecweekly or fortnightly sub
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60 Pawelection, the VERUM Foundatio
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Telomeres and Replicative Senescenc
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Detection of Molecular Events 715De
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Detection of Molecular Events 73hav
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Detection of Molecular Events 75Fig
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Detection of Molecular Events 772.
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Detection of Molecular Events 79Fig
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Detection of Molecular Events 814.
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Page 82 and 83: 86 Kirk and Millerexperiments on un
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Page 112 and 113: Xenobiotic-Metabolizing Enzymes 119
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Page 134 and 135: Comet Assay of DNA Damage 14310Meas
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Page 150 and 151: 160 van der Schanswithin 1 h, after
Page 152 and 153: 162 van der Schans10. High-binding
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Page 156 and 157: 166 van der SchansThe calculations
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Page 176 and 177: 190 HouThe HPRT mutational spectrum
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194 Hou4. Reading: Load 10 µL of t
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196 Hou4.2. MP-PCRUse preferably DN
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Susceptibility of LDL to Oxidation
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Analysis of Pentosidine 20916Measur
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Analysis of Pentosidine 211Fig. 2.
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Analysis of Pentosidine 213Fig. 4.
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Analysis of Pentosidine 2152. Preci
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Analysis of Pentosidine 2177. Nagar
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222 Miquellipoperoxides and malonal
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224 Miquelat 4°C. Protect this sol
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226 Miquel3.1. Animal AnesthesiaMed
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228 Miquelsteps to carry out a conv
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230 Miquelsuspending it over the so
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232 Miquelcontrast, washing with bu
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234 MiquelFig. 3. Electron microsco
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Damage to Mitochondria 23718Causes
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Damage to Mitochondria 239Fig. 1. E
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Damage to Mitochondria 2412. To obt
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Damage to Mitochondria 243synthesis
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Mitochondrial DNA Mutations 24519An
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Mitochondrial DNA Mutations 247F.P.
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Mitochondrial DNA Mutations 249desi
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Mitochondrial DNA Mutations 2514. M
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Mitochondrial DNA Mutations 253Fig.
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Mitochondrial DNA Mutations 25568°
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Mitochondrial DNA Mutations 257Fig.
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Mitochondrial DNA Mutations 2593. P
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Mitochondrial DNA Mutations 261X-10
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Mitochondrial DNA Mutations 26315.
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Mitochondrial DNA Mutations 26520An
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Mitochondrial DNA Mutations 267Tabl
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Mitochondrial DNA Mutations 269muta
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Mitochondrial DNA Mutations 2717. P
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Mitochondrial DNA Mutations 2733.2.
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Mitochondrial DNA Mutations 275that
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Mitochondrial DNA Mutations 27711.
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282 BeckmanWhatever the mechanism e
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284 BeckmanT-cell adherence to vasc
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286 BeckmanT3 hybridoma cells satis
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288 BeckmanHaving identified a cand
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290 Beckmanthe plates vigorously wi
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292 Grubeck-Loebenstein, Saurwein-T
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NK Cell Function in Aging 31123Age-
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NK Cell Function in Aging 3135. Pet
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NK Cell Function in Aging 31518. Io
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NK Cell Function in Aging 3173.3. C
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NK Cell Function in Aging 3199mL of
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Immunogenetics and Life-Span 32124I
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Immunogenetics and Life-Span 323Tab
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Immunogenetics and Life-Span 325rea
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Immunogenetics and Life-Span 3273.
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Immunogenetics and Life-Span 3313.5
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Immunogenetics and Life-Span 349to
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354 Yuto-implement intervention and
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356 Yuof their quality control proc
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358 Yugenesis of major diseases (1-
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Genetically Engineered Mice 36126Th
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Genetically Engineered Mice 365in b
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