Aging Aging

346 Middleton, Curran, and WilliamsTable 12 (cont.)Designation of groups:1.1 0101 01021 010415.2 15011 15012 15021 15022 1504 1505 150616.1 16011 16012 16021 16022 1603 16053.1 03011 03021 0303 0305 0306 03073.2 03012 03022 03114.2 0402 04144.3 04011 04012 0413 0416 04214.5 0403 0406 0407 04204.6 0411 0417 04244.7 0404 0408 0419 04234.8 04051 04052 041011.2 1102 1103 1111 1114 112111.4 11011 11012 11013 11041 11042 1106 11081 11082 1110 11121113 1115 1118 1119 1124 1127 112911.16 1116 112012.1 1201 12021 12022 12031 1203213.2 1301 1302 1316 1320 1328 132913.3 1307 1311 1314 132513.5 1305 1306 130913.6 1312 1321 133013.8 1308 1322 1323 132414.1 1401 140714.3 1403 1412 142714.4 1402 1406 142914.5 1405 1408 141414.7 1409 14178.2 0801 08031 08032 0805 0806 0810 0812 0814 08168.3 08021 08022 08041 08042 0807 0809 0811 0813 0815if a laboratory is setting up a technique from scratch it should ensure that there isno differential amplification by testing various combinations of alleles.6. Many of the probes used in this laboratory are DIG-labeled during their manufacture,adding the DIG moiety to 5' amino oligonucleotides by incubating with aDIG ester under mild alkali conditions.7. A commercial method similar to that described is now available (Lifecodes Corporation,Stamford, CT, USA) whereby probes are supplied already labeled withalkaline phosphatase. This removes the DIG labeling of probes and the use ofanti-DIG antibody.8. When we implement new probes to the system we will initially use a wash temperaturethat is equivalent to the melting temperature of the probes. This is equal