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Dendritic Cells in Old Age 30951. Schuler, G., Lutz, M. B., Bender, A., Röder, C., and Romani, N. (1999) A guide tothe isolation and propagation of dendritic cells, in Dendritic Cells: Biology andClinical Applications (Lotze M. T. and Thomson, A. W., eds.), Adcademic Press,London, Sect. 27, 515–533.52. Koch, F., Kämpgen, E., Schuler, G., and Romani, N (1999) Isolation, enrichmentand culture of murine epidermal Langerhans cells, in Dendritic Cell Protocols —Methods in Molecular Medicine (Robinson, S. and Stagg, A. J., eds.), HumanaPress, Totowa, NJ, in press.53. Inaba, K., Swiggard, W. J., Steinman, R. M., Romani, N., and Schuler, G. (1998)Isolation of dendritic cells, in Current Protocols in Immunology (Coligan, J. E.,Kruisbeek, A. M., Margulies, D. H., Shevach, E. M., and Strober, W., eds.), JohnWiley & Sons, New York, Sect. 3.7.1–3.7.15.54. Rieser, C., Böck, G., Klocker, H., Bartsch, G., and Thurnher, M. (1997) ProstaglandinE2 and tumor necrosis factor α cooperate to activate human dendritic cells:synergistic activation of interleukin 12 production. J. Exp. Med. 196, 1603–1608.55. Jonuleit, H., Kühn, U., Müller, G., Steinbrink, K., Paragnik, L., Schmitt, E., Knop,J., and Enk, A. H. (1997) Pro-inflammatory cytokines and prostaglandins inducematuration of potent immunostimulatory dendritic cells under fetal calf serum-freeconditions. Eur. J. Immunol. 27, 3135–3142.56. Schuler, G. and Steinman, R. M. (1985) Murine epidermal Langerhans cells matureinto potent immunostimulatory dendritic cells in vitro. J. Exp. Med. 161, 526–546.57. Kämpgen, E., Koch, N., Koch, F., Stoger, P., Heufler, C., Schuler, G., and Romani,N. (1991) Class II major histocompatibility complex molecules of murine dendriticcells: synthesis, sialylation of invariant chain, and antigen processing capacity aredown-regulated upon culture. Proc. Natl. Acad. Sci. USA 88, 3014–3018.58. Pope, M., Betjes, M. G. H., Hirmand, H., Hoffman, L, and Steinman, R. M. (1995)Both dendritic cells and memory T lymphocytes emigrate from organ cultures ofhuman skin and form distinctive dendritic- T-cell conjugates. J. Invest. Dermatol.104, 11–17.59. Romani, N., Gruner, S., and Brang, D. (1994) Proliferating dendritic cells progenitorsin human blood. J. Exp. Med. 180, 83–93.60. Inaba, K., Inaba, M., Deguchi, M., Hagi, K., Yasumizu, R., Ikehara, S., Muramatsu,S., and Steinman, R. M. (1993) Granulocytes, macrophages, and dendritic cellsarise from a common major histocompatibility complex class II-negative progenitorin mouse bone marrow. Proc. Natl. Acad. Sci. USA 90, 3038–3042.

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