Aging Aging

Immunogenetics and Life-Span 337Table 9HLA-A SSOP PatternsProbes A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 1HLA-A alleles0101 + + + + + + +0102 + + + + + +2.1 a + + + + + + +0202 + + + + + + +0203 + + + + + + + + +0204/17 + + + + + +0205/08 + + + + + +0206/10/21 + + + + + +0211 + + + + + + + (+)0212/13 + + + + + + + +0214 + + + + +0216 + + + + + + +0219 + + + + + + +0222 + + + + + + + +0301/03N + + + + + +0302 + + + + + +1101/02/03 + + + + +1104 + + + + +2301 + + + + +2402/03/05 + + + + +2404 + + + + + +2406 + + + + +2407 + + + +2408 + + + + +2409N + + + + +2410 + + + + +2413 + + + +2414 + + + + + +2501 + + + + + + + +2502 + + + + + + +(continued)the bench too long (max 15 min — Taq loses activity once diluted in buffer).Switch on the PCR machine for at least 10 min prior to use to allow the machineto heat up. PCR machine should be situated in the post-PCR room.3. After setting up a PCR, wash work areas with sodium hypochlorite (containing2% chlorine). Soak all racks used to hold samples in sodium hypochlorite forapprox 30 min, and rinse thoroughly in water. Pipets should be wiped with sodiumhypochlorite, followed by ddH 2 O. Wipe microcentrifuge, vortex-mix, freezer