Aging Aging

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166 van der SchansThe calculations are carried out only for those dilutions for which a linearrelationship is observed between the amount of DNA in the wells and the levelof fluorescence. In daily practice, this holds for fluorescence values
Immunochemical Assay for DNA Damage 1678. Sometimes the use of the plate washer, and/or the 96-well dispenser presentsproblems owing to (1) contamination of the washer with high amounts ofD1B-AP and (2) contamination or leakage of the dispenser. In that case, the solutionsshould be added manually, with the 12-channel pipet, and the plates shouldbe washed with the hand wash apparatus. The latter is preferred, at least for thelast wash step before adding the reaction buffer.9. Historically, before adding substrate in reaction buffer, the plates were washedonce with 0.1 M diethanolamine, pH 9.8. Later on this step was omitted, as higherfluorescence values without substantial increase of the background fluorescencewere obtained.10. Both the required antibody, D1B, as well as its conjugate, D1B-AP, are madeavailable on request to the author at a cost necessary to cover production, development,and shipment.References1. Van der Schans, G. P., Van Loon, A. A. W. M., Groenendijk, R. H., and Baan, R. A.(1989) Detection of DNA damage in cells exposed to ionizing radiation by use ofanti-single-stranded-DNA monoclonal antibody. Int. J. Radiat. Biol. 55, 747–760.2. Timmerman, A. J., Mars-Groenendijk, R. H., Van der Schans, G. P., and Baan, R. A.(1995) A modified immunochemical assay for the detection of DNA damage inhuman white blood cells. Mutat. Res. 334, 347–356.3. King, C. M., Bristow-Craig, H. E., Gillespie, E. S., and Barnett, Y. A. (1995) In vivoantioxidant status, DNA damage, mutation and DNA repair capacity in culturedlymphocytes from healthy 75- to 80-year-old humans. Mutat. Res. 377, 137–147.4. Van der Schans, G. P. (1993) Method for detecting single-strand breaks in DNA.European patent request, no. 93201672.8, 10 June 1993.
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6 Strehlerlead to possible death si
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Understanding Aging 9the intestine
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Understanding Aging 117. Do long-li
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Understanding Aging 13is, the great
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Understanding Aging 15While this re
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Understanding Aging 172. Pearl, R.
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Understanding Aging 1945. Perovic,
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24 Cristofalo, Volker, and Allenmen
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26 Cristofalo, Volker, and Allenrec
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28 Cristofalo, Volker, and Allenski
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30 Cristofalo, Volker, and AllenTab
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34 Cristofalo, Volker, and AllenNa
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38 Cristofalo, Volker, and Allentak
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40 Cristofalo, Volker, and Allenb.
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42 Cristofalo, Volker, and Allenand
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44 Cristofalo, Volker, and AllenRef
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46 Cristofalo, Volker, and Allen33.
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48 Cristofalo, Volker, and Allen61.
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50 Cristofalo, Volker, and Allenhum
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52 Cristofalo, Volker, and Allen125
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54 Pawelecdiluted cells on an irrad
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56 Pawelec3. Method3.1. Source of C
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58 Pawelecweekly or fortnightly sub
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60 Pawelection, the VERUM Foundatio
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Telomeres and Replicative Senescenc
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Detection of Molecular Events 715De
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Detection of Molecular Events 73hav
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Detection of Molecular Events 75Fig
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Detection of Molecular Events 772.
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Detection of Molecular Events 79Fig
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86 Kirk and Millerexperiments on un
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88 Kirk and MillerFig. 1. (A) Analy
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90 Kirk and MillerFurthermore, limi
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92 Kirk and Millersufficiently dilu
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94 Kirk and Miller4. Run samples on
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96 Kirk and Miller16. Lange-Carter,
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98 Engel, Adibzadeh, and PawelecPCR
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100 Engel, Adibzadeh, and Pawelec5.
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102 Engel, Adibzadeh, and PawelecTa
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104 Engel, Adibzadeh, and PawelecTa
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106 Engel, Adibzadeh, and Pawelecam
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108 Engel, Adibzadeh, and Pawelec3.
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Page 112 and 113: Xenobiotic-Metabolizing Enzymes 119
Page 124 and 125: Assessing Antioxidant Status 1339As
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Page 128 and 129: Assessing Antioxidant Status 137Tab
Page 130 and 131: Assessing Antioxidant Status 139add
Page 132 and 133: Assessing Antioxidant Status 14121.
Page 134 and 135: Comet Assay of DNA Damage 14310Meas
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Page 138 and 139: Comet Assay of DNA Damage 1476. Lys
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Page 150 and 151: 160 van der Schanswithin 1 h, after
Page 152 and 153: 162 van der Schans10. High-binding
Page 154 and 155: 164 van der Schans3. Neutralize aft
Page 158 and 159: 8-oxoguanine Levels in Nuclear DNA
Page 166 and 167: Mutation and the Aging Process 1791
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Page 176 and 177: 190 HouThe HPRT mutational spectrum
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Page 182 and 183: 196 Hou4.2. MP-PCRUse preferably DN
Page 184 and 185: Susceptibility of LDL to Oxidation
Page 194 and 195: Analysis of Pentosidine 20916Measur
Page 196 and 197: Analysis of Pentosidine 211Fig. 2.
Page 198 and 199: Analysis of Pentosidine 213Fig. 4.
Page 200 and 201: Analysis of Pentosidine 2152. Preci
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Page 204 and 205: 222 Miquellipoperoxides and malonal
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224 Miquelat 4°C. Protect this sol
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226 Miquel3.1. Animal AnesthesiaMed
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228 Miquelsteps to carry out a conv
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230 Miquelsuspending it over the so
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232 Miquelcontrast, washing with bu
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234 MiquelFig. 3. Electron microsco
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Damage to Mitochondria 23718Causes
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Damage to Mitochondria 239Fig. 1. E
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Damage to Mitochondria 2412. To obt
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Damage to Mitochondria 243synthesis
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Mitochondrial DNA Mutations 24519An
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Mitochondrial DNA Mutations 247F.P.
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Mitochondrial DNA Mutations 249desi
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Mitochondrial DNA Mutations 2514. M
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Mitochondrial DNA Mutations 253Fig.
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Mitochondrial DNA Mutations 25568°
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Mitochondrial DNA Mutations 257Fig.
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Mitochondrial DNA Mutations 2593. P
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Mitochondrial DNA Mutations 261X-10
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Mitochondrial DNA Mutations 26315.
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Mitochondrial DNA Mutations 26520An
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Mitochondrial DNA Mutations 267Tabl
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Mitochondrial DNA Mutations 269muta
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Mitochondrial DNA Mutations 2717. P
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Mitochondrial DNA Mutations 2733.2.
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Mitochondrial DNA Mutations 275that
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Mitochondrial DNA Mutations 27711.
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282 BeckmanWhatever the mechanism e
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284 BeckmanT-cell adherence to vasc
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286 BeckmanT3 hybridoma cells satis
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288 BeckmanHaving identified a cand
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290 Beckmanthe plates vigorously wi
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292 Grubeck-Loebenstein, Saurwein-T
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NK Cell Function in Aging 31123Age-
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NK Cell Function in Aging 3135. Pet
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NK Cell Function in Aging 31518. Io
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NK Cell Function in Aging 3173.3. C
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NK Cell Function in Aging 3199mL of
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Immunogenetics and Life-Span 32124I
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Immunogenetics and Life-Span 323Tab
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Immunogenetics and Life-Span 325rea
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Immunogenetics and Life-Span 3273.
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Immunogenetics and Life-Span 3313.5
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Immunogenetics and Life-Span 349to
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354 Yuto-implement intervention and
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356 Yuof their quality control proc
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358 Yugenesis of major diseases (1-
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Genetically Engineered Mice 36126Th
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Genetically Engineered Mice 363as t
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Genetically Engineered Mice 365in b
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Genetically Engineered Mice 367numb
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Genetically Engineered Mice 369mula
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Genetically Engineered Mice 371Othe
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Genetically Engineered Mice 373locu
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Genetically Engineered Mice 37537.
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Genetically Engineered Mice 377→
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Aging Aging

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