Aging Aging

76 Carmody et al.Fig. 2. (A) Schematic diagram of mitochondrial events during apoptosis. Intactmitochondria display high transmembrane potential (∆ψ m ) and fluoresce strongly inFL-2 when stained with JC-1. Apoptotic mitochondria undergo permeability transitionresulting in a release of solutes 1.5 KDa and smaller and a loss transmembrane potential,consequently displaying reduced fluorescence in FL-2 when stained with JC-1.(B) Alterations in ∆ψ m in NSO myeloma cells upon camptothecin treatment. NSOmyeloma cells were treated with 30 mM ammonia for 18 h. Depolarization in ∆ψ m wasassessed as described in Subheading 2.3., using JC-1. ∆ψ m depolarization can bemonitored by measuring the fluorescence in FL-1. Membrane depolarization results inan increase in FL-1 flourescence. Treated cells (solid line) show an increase in FL-1fluorescence relative to untreated cells (dashed line).