Aging Aging

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Immunogenetics and Life-Span 345Table 12 (cont.)Probe 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 22 23 24 25 26HLA-DR allele13 13.2 + + + + +13.3 + + +13.5 + + + +13.6 + + + +13.8 + + + +13031/32 + + + + +1304 + + + + +1310 + + + + +1313 + + + + +1315/27 + + + +1317 + + + +1318 + + + + +1319 + + +1326 + + +14 14.1 + + + + +14.3 + + + +14.4 + + + +14.5 + + + +14.7 + + + + +1404/28 + + + + +1410 + + + + +1411 + + + + +1413 + + + + +1415 + + + +1416 + + + + +1418 + + + +1419 + + + +1420 + + +1421 + + + + +1422/25 + + + +1423 + + + +1424 + + +1426 + + + +7 0701 + + + +8 8.2 + + + + +8.3 + + + +0808 + + + + +9 09012 + + + +10 1001 + +The alleles listed are those identified in HLA-DR sequences from ASHI, April 1997.
346 Middleton, Curran, and WilliamsTable 12 (cont.)Designation of groups:1.1 0101 01021 010415.2 15011 15012 15021 15022 1504 1505 150616.1 16011 16012 16021 16022 1603 16053.1 03011 03021 0303 0305 0306 03073.2 03012 03022 03114.2 0402 04144.3 04011 04012 0413 0416 04214.5 0403 0406 0407 04204.6 0411 0417 04244.7 0404 0408 0419 04234.8 04051 04052 041011.2 1102 1103 1111 1114 112111.4 11011 11012 11013 11041 11042 1106 11081 11082 1110 11121113 1115 1118 1119 1124 1127 112911.16 1116 112012.1 1201 12021 12022 12031 1203213.2 1301 1302 1316 1320 1328 132913.3 1307 1311 1314 132513.5 1305 1306 130913.6 1312 1321 133013.8 1308 1322 1323 132414.1 1401 140714.3 1403 1412 142714.4 1402 1406 142914.5 1405 1408 141414.7 1409 14178.2 0801 08031 08032 0805 0806 0810 0812 0814 08168.3 08021 08022 08041 08042 0807 0809 0811 0813 0815if a laboratory is setting up a technique from scratch it should ensure that there isno differential amplification by testing various combinations of alleles.6. Many of the probes used in this laboratory are DIG-labeled during their manufacture,adding the DIG moiety to 5' amino oligonucleotides by incubating with aDIG ester under mild alkali conditions.7. A commercial method similar to that described is now available (Lifecodes Corporation,Stamford, CT, USA) whereby probes are supplied already labeled withalkaline phosphatase. This removes the DIG labeling of probes and the use ofanti-DIG antibody.8. When we implement new probes to the system we will initially use a wash temperaturethat is equivalent to the melting temperature of the probes. This is equal
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6 Strehlerlead to possible death si
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Understanding Aging 9the intestine
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Understanding Aging 117. Do long-li
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Understanding Aging 13is, the great
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Understanding Aging 15While this re
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Understanding Aging 172. Pearl, R.
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Understanding Aging 1945. Perovic,
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24 Cristofalo, Volker, and Allenmen
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26 Cristofalo, Volker, and Allenrec
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28 Cristofalo, Volker, and Allenski
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30 Cristofalo, Volker, and AllenTab
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34 Cristofalo, Volker, and AllenNa
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38 Cristofalo, Volker, and Allentak
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40 Cristofalo, Volker, and Allenb.
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42 Cristofalo, Volker, and Allenand
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44 Cristofalo, Volker, and AllenRef
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46 Cristofalo, Volker, and Allen33.
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48 Cristofalo, Volker, and Allen61.
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50 Cristofalo, Volker, and Allenhum
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52 Cristofalo, Volker, and Allen125
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54 Pawelecdiluted cells on an irrad
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56 Pawelec3. Method3.1. Source of C
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58 Pawelecweekly or fortnightly sub
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60 Pawelection, the VERUM Foundatio
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Telomeres and Replicative Senescenc
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Detection of Molecular Events 715De
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Detection of Molecular Events 73hav
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Detection of Molecular Events 75Fig
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Detection of Molecular Events 772.
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Detection of Molecular Events 79Fig
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86 Kirk and Millerexperiments on un
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88 Kirk and MillerFig. 1. (A) Analy
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90 Kirk and MillerFurthermore, limi
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92 Kirk and Millersufficiently dilu
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94 Kirk and Miller4. Run samples on
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96 Kirk and Miller16. Lange-Carter,
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98 Engel, Adibzadeh, and PawelecPCR
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100 Engel, Adibzadeh, and Pawelec5.
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102 Engel, Adibzadeh, and PawelecTa
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104 Engel, Adibzadeh, and PawelecTa
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106 Engel, Adibzadeh, and Pawelecam
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110 Engel, Adibzadeh, and Pawelecfo
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114 Engel, Adibzadeh, and Pawelecha
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Xenobiotic-Metabolizing Enzymes 119
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Assessing Antioxidant Status 1339As
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Assessing Antioxidant Status 135Fig
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Assessing Antioxidant Status 137Tab
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Assessing Antioxidant Status 139add
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Assessing Antioxidant Status 14121.
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Comet Assay of DNA Damage 14310Meas
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Comet Assay of DNA Damage 145contro
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Comet Assay of DNA Damage 1476. Lys
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Comet Assay of DNA Damage 1494. Gen
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Comet Assay of DNA Damage 151anode
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Comet Assay of DNA Damage 1535. As
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Comet Assay of DNA Damage 15515. In
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Comet Assay of DNA Damage 15720. Co
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160 van der Schanswithin 1 h, after
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162 van der Schans10. High-binding
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164 van der Schans3. Neutralize aft
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166 van der SchansThe calculations
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8-oxoguanine Levels in Nuclear DNA
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Mutation and the Aging Process 1791
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Mutation and the Aging Process 181m
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Mutation and the Aging Process 183b
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190 HouThe HPRT mutational spectrum
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192 Houamplifications are carried o
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194 Hou4. Reading: Load 10 µL of t
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196 Hou4.2. MP-PCRUse preferably DN
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Susceptibility of LDL to Oxidation
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Analysis of Pentosidine 20916Measur
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Analysis of Pentosidine 211Fig. 2.
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Analysis of Pentosidine 213Fig. 4.
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Analysis of Pentosidine 2152. Preci
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Analysis of Pentosidine 2177. Nagar
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222 Miquellipoperoxides and malonal
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224 Miquelat 4°C. Protect this sol
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226 Miquel3.1. Animal AnesthesiaMed
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228 Miquelsteps to carry out a conv
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230 Miquelsuspending it over the so
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232 Miquelcontrast, washing with bu
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234 MiquelFig. 3. Electron microsco
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Damage to Mitochondria 23718Causes
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Damage to Mitochondria 239Fig. 1. E
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Damage to Mitochondria 2412. To obt
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Damage to Mitochondria 243synthesis
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Mitochondrial DNA Mutations 24519An
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Mitochondrial DNA Mutations 247F.P.
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Mitochondrial DNA Mutations 249desi
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Mitochondrial DNA Mutations 2514. M
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Mitochondrial DNA Mutations 253Fig.
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Mitochondrial DNA Mutations 25568°
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Mitochondrial DNA Mutations 257Fig.
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Mitochondrial DNA Mutations 2593. P
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Mitochondrial DNA Mutations 261X-10
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Mitochondrial DNA Mutations 26315.
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Mitochondrial DNA Mutations 26520An
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Mitochondrial DNA Mutations 267Tabl
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Mitochondrial DNA Mutations 269muta
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Mitochondrial DNA Mutations 2717. P
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Mitochondrial DNA Mutations 2733.2.
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Mitochondrial DNA Mutations 275that
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Mitochondrial DNA Mutations 27711.
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282 BeckmanWhatever the mechanism e
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284 BeckmanT-cell adherence to vasc
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286 BeckmanT3 hybridoma cells satis
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288 BeckmanHaving identified a cand
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290 Beckmanthe plates vigorously wi
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292 Grubeck-Loebenstein, Saurwein-T
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Page 288 and 289: NK Cell Function in Aging 31123Age-
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Page 328 and 329: 354 Yuto-implement intervention and
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Page 334 and 335: Genetically Engineered Mice 36126Th
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Aging Aging

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