Aging Aging

338 Middleton, Curran, and WilliamsTable 9 (cont.)Probes A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 12601/02 + + + + + + + +2603/05/06 + + + + + + +2604 + + + + + + +2607 + + + + + + + +2608 + + + + + + + +68011/012/02 + + + + + + +6803 + + + + + + + +6804 + + + + + + (+) +6805 + + + + + + +6901 + + + + + +2901/02/03 + + + + + +3001 + + + + + +3002 + + + + + +3003 + + + + +3004 + + + + + + +31012 + + + + + (+) +3201 + + + + + + +3202 + + + + + + +3301/03 + + + + + (+) +3401 + + + + + + +3402 + + + + + + +3601 + + + + + + +4301 + + + + + + + +6601 + + + + + + +6602 + + + + + + +6603 + + + + + + + +7401/02/03 + + + + + + +8001 + (+) + + + +The alleles listed are those identified in HLA-A sequences from ASHI, April 1997.a2.1 represents the alleles 0201, 0207, 0215 N, 0218, 0220.A (+) indicates probe is positive in practice but would not appear to be from sequence.handle, etc., with sodium hypochlorite. Expose the working area, including pipetsetc., to UV light for 60 min.4. When performing a PCR on 96 samples there may be one or two samples that arenot amplified. Therefore we always run a gel to ensure that we have product. Thisenables the SSOP method to be well controlled. (If an amplification fails in theSequence Specific Primer method this would lead to an incorrect result.) On someoccasions the product is deemed weak and this sample will always be repeated.Good amplification always gives a clean and clear-cut SSOP hybridizationwhereas almost all the problematic typing results we have encountered were due